The Trypanosoma cruzi Protein TcHTE Is Critical for Heme Uptake

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dc.citation.titlePLoS Neglected Tropical Diseaseses
dc.citation.volume10(1)es
dc.creatorMerli, Marcelo Luciano
dc.creatorPagura, Lucas
dc.creatorHernández, Josefina
dc.creatorBarisón, María Julia
dc.creatorPral, Elizabeth M. F.
dc.creatorSilber, Ariel M.
dc.creatorCricco, Julia Alejandra
dc.date.accessioned2020-06-08T16:51:12Z
dc.date.available2020-06-08T16:51:12Z
dc.date.issued2016-01
dc.descriptionTrypanosoma cruzi, the etiological agent of Chagas' disease, presents nutritional requirements for several metabolites. It requires heme for the biosynthesis of several heme-proteins involved in essential metabolic pathways like mitochondrial cytochromes and respiratory complexes, as well as enzymes involved in the biosynthesis of sterols and unsaturated fatty acids. However, this parasite lacks a complete route for its synthesis. In view of these facts, T. cruzi has to incorporate heme from the environment during its life cycle. In other words, their hosts must supply the heme for heme-protein synthesis. Although the acquisition of heme is a fundamental issue for the parasite’s replication and survival, how this cofactor is imported and distributed is poorly understood. In this work, we used different fluorescent heme analogs to explore heme uptake along the different life-cycle stages of T. cruzi, showing that this parasite imports it during its replicative stages: the epimastigote in the insect vector and the intracellular amastigote in the mammalian host. Also, we identified and characterized a T. cruzi protein (TcHTE) with 55% of sequence similarity to LHR1 (protein involved in L. amazonensis heme transport), which is located in the flagellar pocket, where the transport of nutrients proceeds in trypanosomatids. We postulate TcHTE as a protein involved in improving the efficiency of the heme uptake or trafficking in T. cruzi.es
dc.description.filFil: Merli, Marcelo Luciano. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.es
dc.description.filFil: Pagura, Lucas. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.es
dc.description.filFil: Hernández, Josefina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.es
dc.description.filFil: Barisón, María Julia. Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Parasitologia. Laboratory of Biochemistry of Tryps (LaBTryps); Brasil.es
dc.description.filFil: Pral, Elizabeth M. F. Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Parasitologia. Laboratory of Biochemistry of Tryps (LaBTryps); Brasil.es
dc.description.filFil: Silber, Ariel M. Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Parasitologia. Laboratory of Biochemistry of Tryps (LaBTryps); Brasil.es
dc.description.filFil: Cricco, Julia Alejandra. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química Biológica. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.es
dc.description.sponsorshipAgencia Nacional de Promoción Científica y Tecnológica (ANPCyT): grant PICTO-GSK 2011-0011es
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP): grant 13/18970-6es
dc.description.sponsorshipInstituto Nacional de Biologia Estrutural e Química Medicinal em Doenças Infecciosas (INBEQMeDI)es
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq): grant 475741/2013-7es
dc.formatapplication/pdf
dc.format.extent1-18es
dc.identifier.issn1935-2735es
dc.identifier.urihttp://hdl.handle.net/2133/18325
dc.language.isoenges
dc.publisherPublic Library of Science PLOSes
dc.relation.publisherversionhttps://doi.org/10.1371/journal.pntd.0004359es
dc.relation.publisherversionhttps://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0004359#ackes
dc.rightsopenAccesses
dc.rights.holderMerli, Marcelo Lucianoes
dc.rights.holderPagura, Lucases
dc.rights.holderHernández, Josefinaes
dc.rights.holderBarisón, María Juliaes
dc.rights.holderPral, Elizabeth M. F.es
dc.rights.holderSilber, Ariel M.es
dc.rights.holderCricco, Julia Alejandraes
dc.rights.holderUniversidad Nacional de Rosarioes
dc.rights.textAttribution 4.0 International (CC BY 4.0)es
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectTrypanosoma cruzies
dc.subjectChagas' diseasees
dc.subjectTrypanosoma cruzi Protein TcHTEes
dc.subjectHeme uptakees
dc.subjectParasitic life cycleses
dc.titleThe Trypanosoma cruzi Protein TcHTE Is Critical for Heme Uptakees
dc.typearticle
dc.typeartículo
dc.typepublishedVersion
dc.type.collectionarticulo
dc.type.versionpublishedVersiones

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