Carbapenem‑resistant Acinetobacter baumannii (CRAB): metabolic adaptation and transcriptional response to human urine (HU)

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dc.citation.titleScientific Reports
dc.citation.volume14
dc.creatorEscalante, Jenny
dc.creatorHamza, Mase
dc.creatorNishimura, Brent
dc.creatorMelecio, Meghan
dc.creatorDavies Sala, Carol
dc.creatorTuttobene, Marisel Romina
dc.creatorSubils, Tomás
dc.creatorTraglia, German M.
dc.creatorPham, Chloe
dc.creatorSieira, Rodrigo
dc.creatorActis, Luis A.
dc.creatorBonomo, Robert A.
dc.creatorTolmasky, Marcelo E.
dc.creatorRamírez, María Soledad
dc.date.accessioned2025-03-07T13:53:04Z
dc.date.available2025-03-07T13:53:04Z
dc.date.issued2024-08-19
dc.description.abstractCarbapenem-resistant Acinetobacter baumannii (CRAB) is a major human pathogen and a research priority for developing new antimicrobial agents. CRAB is a causative agent of a variety of infections in diferent body sites. One of the manifestations is catheter-associated urinary tract infection, which exposes the bacteria to the host’s urine, creating a particular environment. Exposure of two CRAB clinical isolates, AB5075 and AMA40, to human urine (HU) resulted in the diferential expression levels of 264 and 455 genes, respectively, of which 112 were common to both strains. Genes within this group play roles in metabolic pathways such as phenylacetic acid (PAA) catabolism, the Hut system, the tricarboxylic acid (TCA) cycle, and other processes like quorum sensing and bioflm formation. These results indicate that the presence of HU induces numerous adaptive changes in gene expression of the infecting bacteria. These changes presumably help bacteria establish and thrive in the hostile conditions in the urinary tract. These analyses advance our understanding of CRAB’s metabolic adaptations to human fuids, as well as expand knowledge on bacterial responses to distinct human fuids containing diferent concentrations of human serum albumin (HSA).
dc.description.filFil: Escalante, Jenny. California State University Fullerton. College of Natural Sciences and Mathematics. Center for Applied Biotechnology Studies. Department of Biological Science; United States.
dc.description.filFil: Hamza, Mase. California State University Fullerton. College of Natural Sciences and Mathematics. Center for Applied Biotechnology Studies. Department of Biological Science; United States.
dc.description.filFil: Nishimura, Brent. California State University Fullerton. College of Natural Sciences and Mathematics. Center for Applied Biotechnology Studies. Department of Biological Science; United States.
dc.description.filFil: Melecio, Meghan. California State University Fullerton. College of Natural Sciences and Mathematics. Center for Applied Biotechnology Studies. Department of Biological Science; United States.
dc.description.filFil: Davies Sala, Carol. California State University Fullerton. College of Natural Sciences and Mathematics. Center for Applied Biotechnology Studies. Department of Biological Science; United States.
dc.description.filFil: Tuttobene, Marisel Romina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Biología Molecular; Argentina.
dc.description.filFil: Tuttobene, Marisel Romina. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Subils, Tomás. Instituto de Procesos Biotecnológicos y Químicos de Rosario (IPROBYQ-CONICET); Argentina.
dc.description.filFil: Traglia, German M. Universidad de la República. Unidad de Genómica y Bioinformática. Departamento de Ciencias Biológicas; Uruguay.
dc.description.filFil: Pham, Chloe. California State University Fullerton. College of Natural Sciences and Mathematics. Center for Applied Biotechnology Studies. Department of Biological Science; United States.
dc.description.filFil: Sieira, Rodrigo. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires (IIBBA-CONICET); Argentina.
dc.description.filFil: Actis, Luis A. Miami University. Department of Microbiology; United States.
dc.description.filFil: Bonomo, Robert A. Research Service and GRECC. Louis Stokes Cleveland Department of Veterans Afairs Medical Center; United States.
dc.description.filFil: Bonomo, Robert A. Case Western Reserve University School of Medicine. Departments of Medicine, Pharmacology, Molecular Biology and Microbiology, Biochemistry, Proteomics and Bioinformatics; United States.
dc.description.filFil: Bonomo, Robert A. CWRU-Cleveland VAMC Center for Antimicrobial Resistance and Epidemiology; United States.
dc.description.filFil: Tolmasky, Marcelo E. California State University Fullerton. College of Natural Sciences and Mathematics. Center for Applied Biotechnology Studies. Department of Biological Science; United States.
dc.description.filFil: Ramírez, María Soledad. California State University Fullerton. College of Natural Sciences and Mathematics. Center for Applied Biotechnology Studies. Department of Biological Science; United States.
dc.description.sponsorshipNational Institutes of Health
dc.description.sponsorshipThe Cleveland Department of Veterans Affairs: 1I01BX001974
dc.description.sponsorshipNational Institute on Minority Health and Health Disparities (NIMHD): MHRT 2T37MD001368
dc.format.extent1-10
dc.identifier.citationEscalante, J., Hamza, M., Nishimura, B. et al. Carbapenem-resistant Acinetobacter baumannii (CRAB): metabolic adaptation and transcriptional response to human urine (HU). Sci Rep 14, 19145 (2024). https://doi.org/10.1038/s41598-024-70216-w
dc.identifier.issn2045-2322
dc.identifier.urihttps://hdl.handle.net/2133/29015
dc.language.isoen
dc.publisherNature Research
dc.relation.publisherversionhttps://doi.org/10.1038/s41598-024-70216-w
dc.relation.publisherversionhttps://www.nature.com/articles/s41598-024-70216-w
dc.rightsopenAccess
dc.rights.holderEscalante, Jenny
dc.rights.holderHamza, Mase
dc.rights.holderNishimura, Brent
dc.rights.holderMelecio, Meghan
dc.rights.holderDavies Sala, Carol
dc.rights.holderTuttobene, Marisel Romina
dc.rights.holderSubils, Tomás
dc.rights.holderTraglia, German M.
dc.rights.holderPham, Chloe
dc.rights.holderSieira, Rodrigo
dc.rights.holderActis, Luis A.
dc.rights.holderBonomo, Robert A.
dc.rights.holderTolmasky, Marcelo E.
dc.rights.holderRamírez, María Soledad
dc.rights.textAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectAcinetobacter baumannii
dc.subjectHuman urine
dc.subjectHuman serum albumin
dc.subjectCarbapenem-resistance
dc.titleCarbapenem‑resistant Acinetobacter baumannii (CRAB): metabolic adaptation and transcriptional response to human urine (HU)
dc.typearticulo
dc.type.collectionarticulo
dc.type.versionpublishedVersion

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