Hepatic preneoplasia induction in male Wistar rats: histological studies up to five months post treatment

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dc.citation.titleRevista Española de Enfermedades Digestivas (REED)
dc.citation.volume108
dc.creatorPisani, Gerardo Bruno
dc.creatorValenti, José Luis
dc.creatorQuintana, Alejandra Beatriz
dc.date.accessioned2024-07-03T13:29:40Z
dc.date.available2024-07-03T13:29:40Z
dc.date.issued2016-08
dc.description.abstractBackground: Liver preneoplasia development in rats can be mimicked by an initiation-promotion model that induces the appearance of altered hepatocyte foc (FAH). Aims: We compare two initiation-promotion models to evaluate the presence of FAH or additional hepatic pathologies in which other organs were affected up to five month post treatment. Material and methods: FAH were induced in male adult Wistar rats with two doses of dietylnitrosamine (DEN, 150 mg/kg bw) followed by 4 doses per week (3 weeks) of 2-acetylaminofluorene (2-AAF, 20 mg/kg bw) or with one dose of DEN (200 mg/kg bw) followed by 2 doses per week (3 weeks) of 2-AAF. DEN 150, DEN 200 and control mice (received the vehicle of the drugs) groups were compared. Rats were euthanized immediately after the last dose of 2-AAF, at 3, 4 and 5 months (n = 3 euthanasia times per group). Samples of livers, lungs, kidneys, pancreatic tissue and small bowel were processed for histological and immunohistochemical analysis. Results: FAH persisted for 5 months in all livers of the DEN groups. Three months after withdrawal of 2-AAF, one rat from the DEN 150 group developed fibrosis and 5 months after 2-AAF removal another rat from the same group presented a microscopic hyperplastic nodule. Only the lungs had damage compatible with lesions induced by gavage-related reflux in DEN groups. Conclusion: We concluded that up to five month post treatments, FAH persisted in all the livers from the DEN groups; livers from the DEN 200 group showed no other hepatic lesions besides FAH, and only the lungs suffered pathological alterations in both treated groups.
dc.description.filFil: Pisani, Gerardo Bruno. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Fisiología. Área de Morfología; Argentina.
dc.description.filFil: Quintana, Alejandra Beatriz. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Fisiología. Área de Morfología; Argentina.
dc.description.filFil: Valenti, José Luis. Universidad Abierta Interamericana. Facultad de Medicina y Ciencias de la Salud. Área de Patología; Argentina.
dc.description.sponsorshipSecretaría de Ciencia, Tecnología e Innovación de la provincia de Santa Fe (SeCTeI): Project No. 2010-093-11
dc.description.versionpeerreviewed
dc.format.extent1-7
dc.identifier.issn1130-0108
dc.identifier.urihttps://hdl.handle.net/2133/27404
dc.language.isoen
dc.publisherSociedad Española de Patología Digestiva (SEPD)
dc.relation.publisherversionhttps://doi.org/10.17235/reed.2016.4183/2015
dc.relation.publisherversionhttps://www.reed.es/hepatic-preneoplasia-induction-in-male-wistar-rats-histological-studies-up-to-five-months-post-treatment1057
dc.rightsopenAccess
dc.rights.holderPisani, Gerardo Bruno
dc.rights.holderValenti, José Luis
dc.rights.holderQuintana, Alejandra Beatriz
dc.rights.holderUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas
dc.rights.textAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectDiethilnitrosamine
dc.subject2-acetylaminofluorene
dc.subjectRat liver preneoplasia
dc.subjectFoci of altered hepatocytes
dc.subjectReflux
dc.titleHepatic preneoplasia induction in male Wistar rats: histological studies up to five months post treatment
dc.typearticulo
dc.type.collectionarticulo
dc.type.versionpublishedVersion

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