Endocannabinoid 2-arachidonoyl glycerol increases the transcription of daf-7 in ASI neurons

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dc.citation.titlemicroPublication Biology
dc.creatorGalles, Celina
dc.creatorPrez, Gastón M.
dc.creatorDe Mendoza, Diego
dc.date.accessioned2024-04-24T19:23:47Z
dc.date.available2024-04-24T19:23:47Z
dc.date.issued2018-10-19
dc.description.abstractThis article investigates the effect of the endocannabinoid 2-arachidonoyl glycerol (2-AG) on the expression of the daf-7 gene in ASI neurons of nematodes. It demonstrates that daf-7 expression levels increase notably when nematodes are exposed to 2-AG. This observation is supported by fluorescence microscopy images showing elevated GFP signal in ASI neurons treated with 2-AG compared to control groups. Statistical analysis confirms a significant difference in daf-7 expression between the 2-AG treatment and solvent control conditions. The study conducted three independent experiments, analyzing a total of 70 ASI neurons for each condition. Regarding the methods, worms were cultured on solid NGM plates supplemented with bacteria and specific supplements for each experimental condition. Daf-7 expression was monitored in synchronized L1 larvae fed with Escherichia coli supplemented with either 2-AG or carrier solvent. Fluorescence microscopy was utilized to visualize GFP intensity in ASI neurons, and image analysis software quantified the data. The study concludes a robust increase in daf-7 expression in response to 2-AG treatment.
dc.description.filFil: Galles, Celina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET). Laboratorio de Fisiología Microbiana; Argentina.
dc.description.filFil: Prez, Gastón M. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET). Laboratorio de Fisiología Microbiana; Argentina.
dc.description.filFil: De Mendoza, Diego. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET). Laboratorio de Fisiología Microbiana; Argentina.
dc.description.sponsorshipAgencia Nacional de Promoción de la Investigación, el Desarrollo Tecnológico y la Innovación (Agencia I+D+i): PICT 2155, PICT 3693
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET)
dc.description.sponsorshipFundación Bunge y Born
dc.description.versionpeerreviewed
dc.format.extent1-2
dc.identifier.issn2578-9430
dc.identifier.urihttps://hdl.handle.net/2133/26938
dc.language.isoen
dc.publisherCaltech Library
dc.relation.publisherversionhttps://doi.org/10.17912/micropub.biology.000056
dc.relation.publisherversionhttps://www.micropublication.org/journals/biology/micropub-biology-000056
dc.rightsopenAccess
dc.rights.holderGalles, Celina
dc.rights.holderPrez, Gastón M.
dc.rights.holderDe Mendoza, Diego
dc.rights.holderUniversidad Nacional de Rosario
dc.rights.textAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject2-arachidonoyl glycerol
dc.subjectDaf-7
dc.subjectASI neurons
dc.subjectNematodes
dc.subjectStrain FK181 ksIs2
dc.subjectAcetonitrile
dc.titleEndocannabinoid 2-arachidonoyl glycerol increases the transcription of daf-7 in ASI neurons
dc.typearticulo
dc.type.collectionarticulo
dc.type.versionpublishedVersion

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(FBIOyF) Departamento de Química Orgánica - Artículos