Sequence characterization, in silico mapping and cytosine methylation analysis of markers linked to apospory in Paspalum notatum

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dc.citation.titleGenetics and Molecular Biologyes
dc.citation.volume35es
dc.creatorPodio, Maricel
dc.creatorRodríguez, María Pía
dc.creatorFelitti, Silvina
dc.creatorStein, Juliana
dc.creatorMartínez, Erci Javier
dc.creatorSiena, Lorena Adelina
dc.creatorQuarin, Camilo Luis
dc.creatorPessino, Silvina Claudia
dc.creatorOrtiz, Juan Pablo Amelio
dc.date.accessioned2018-12-11T14:13:58Z
dc.date.available2018-12-11T14:13:58Z
dc.date.issued2012-07-01
dc.descriptionIn previous studies we reported the identification of several AFLP, RAPD and RFLP molecular markers linked to apospory in Paspalum notatum . The objective of this work was to sequence these markers, obtain their flanking re -gions by chromosome walking and perform an in silico mapping analysis in rice and maize. The methylation status of two apospory-related sequences was also assessed using methylation-sensitive RFLP experiments. Fourteen mo -lecular markers were analyzed and several protein-coding sequences were identified. Copy number estimates and RFLP linkage analysis showed that the sequence PnMAI3 displayed 2-4 copies per genome and linkage to apospory. Extension of this marker by chromosome walking revealed an additional protein-coding sequence map- ping in silico in the apospory-syntenic regions of rice and maize. Approximately 5 kb corresponding to different mark- ers were characterized through the global sequencing procedure. A more refined analysis based on sequence information indicated synteny with segments of chromosomes 2 and 12 of rice and chromosomes 3 and 5 of maize. Two loci associated with apomixis locus were tested in methylation-sensitive RFLP experiments using genomic DNA extracted from leaves. Although both target sequences were methylated no methylation polymorphisms associated with the mode of reproduction were detected.es
dc.description.filLaboratorio de Biología Molecular, Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, Zavalla, Santa Fe, Argentinaes
dc.formatapplication/pdf
dc.format.extent827-837es
dc.identifier.issn1415-4757es
dc.identifier.urihttp://hdl.handle.net/2133/13785
dc.language.isoenges
dc.publisherSociedade Brasileira de Genéticaes
dc.relation.publisherversionwww.gmb.org.bres
dc.rightsopenAccesses
dc.rights.holderAutoreses
dc.rights.textCreative Commons Legal Code Attribute 2.5 (http://creativecommons.org/licenses/by/2.5/legalcode)es
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/deed.es*
dc.subjectapomixises
dc.subjectchromosome walkinges
dc.subjectgene mappinges
dc.subjectmolecular markerses
dc.titleSequence characterization, in silico mapping and cytosine methylation analysis of markers linked to apospory in Paspalum notatumes
dc.typearticle
dc.typeartículo
dc.typepublishedVersion
dc.type.collectionarticulo
dc.type.versionpublishedVersiones

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