Cerebrospinal fuid (CSF) augments metabolism and virulence expression factors in Acinetobacter baumannii

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dc.citation.titleScientific Reports
dc.citation.volume11
dc.creatorMartínez, Jasmine
dc.creatorRazo Gutiérrez, Chelsea
dc.creatorRazo Gutiérrez, Chelsea
dc.creatorCourville, Robert
dc.creatorPimentel, Camila
dc.creatorLiu, Christine
dc.creatorFung, Sammie E.
dc.creatorTuttobene, Marisel Romina
dc.creatorPhan, Kimberly
dc.creatorVila, Alejandro J.
dc.creatorShahrestani, Parvin
dc.creatorJimenez, Veronica
dc.creatorTolmasky, Marcelo E.
dc.creatorBecka, Scott A.
dc.creatorPapp Wallace, Krisztina M.
dc.creatorBonomo, Robert A.
dc.creatorSoler Bistue, Alfonso
dc.creatorSieira, Rodrigo
dc.creatorRamírez, María Soledad
dc.date.accessioned2024-12-13T15:31:46Z
dc.date.available2024-12-13T15:31:46Z
dc.date.issued2021-02-26
dc.description.abstractIn a recent report by the Centers for Disease Control and Prevention (CDC), multidrug resistant (MDR) Acinetobacter baumannii is a pathogen described as an “urgent threat.” Infection with this bacterium manifests as diferent diseases such as community and nosocomial pneumonia, bloodstream infections, endocarditis, infections of the urinary tract, wound infections, burn infections, skin and soft tissue infections, and meningitis. In particular, nosocomial meningitis, an unwelcome complication of neurosurgery caused by extensivelydrug resistant (XDR) A. baumannii, is extremely challenging to manage. Therefore, understanding how A. baumannii adapts to diferent host environments, such as cerebrospinal fuid (CSF) that may trigger changes in expression of virulence factors that are associated with the successful establishment and progress of this infection is necessary. The present invitro work describes, the genetic changes that occur during A. baumannii infltration into CSF and displays A. baumannii’s expansive versatility to persist in a nutrient limited environment while enhancing several virulence factors to survive and persist. While a hypervirulent A. baumannii strain did not show changes in its transcriptome when incubated in the presence of CSF, a lowvirulence isolate showed signifcant diferences in gene expression and phenotypic traits. Exposure to 4% CSF caused increased expression of virulence factors such as fmbriae, pilins, and iron chelators, and other virulence determinants that was confrmed in various model systems. Furthermore, although CSF’s presence did not enhance bacterial growth, an increase of expression of genes encoding transcription, translation, and the ATP synthesis machinery was observed. This work also explores A. baumannii’s response to an essential component, human serum albumin (HSA), within CSF to trigger the diferential expression of genes associated with its pathoadaptibility in this environment.
dc.description.filFil: Martínez, Jasmine. California State University Fullerton. College of Natural Sciences and Mathematics. Center for Applied Biotechnology Studies. Department of Biological Science; USA.
dc.description.filFil: Razo Gutiérrez, Chelsea. California State University Fullerton. College of Natural Sciences and Mathematics. Center for Applied Biotechnology Studies. Department of Biological Science; USA.
dc.description.filFil: Le, Casin. California State University Fullerton. College of Natural Sciences and Mathematics. Center for Applied Biotechnology Studies. Department of Biological Science; USA.
dc.description.filFil: Courville, Robert. California State University Fullerton. College of Natural Sciences and Mathematics. Center for Applied Biotechnology Studies. Department of Biological Science; USA.
dc.description.filFil: Pimentel, Camila. California State University Fullerton. College of Natural Sciences and Mathematics. Center for Applied Biotechnology Studies. Department of Biological Science; USA.ç
dc.description.filFil: Liu, Christine. California State University Fullerton. College of Natural Sciences and Mathematics. Center for Applied Biotechnology Studies. Department of Biological Science; USA.
dc.description.filFil: Fung, Sammie E. California State University Fullerton. College of Natural Sciences and Mathematics. Center for Applied Biotechnology Studies. Department of Biological Science; USA.
dc.description.filFil: Tuttobene, Marisel Romina. California State University Fullerton. College of Natural Sciences and Mathematics. Center for Applied Biotechnology Studies. Department of Biological Science; USA.
dc.description.filFil: Phan, Kimberly. California State University Fullerton. College of Natural Sciences and Mathematics. Center for Applied Biotechnology Studies. Department of Biological Science; USA.
dc.description.filFil: Vila, Alejandro J. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Vila, Alejandro J. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Biofísica; Argentina.
dc.description.filFil: Shahrestani, Parvin. California State University Fullerton. College of Natural Sciences and Mathematics. Center for Applied Biotechnology Studies. Department of Biological Science; USA.
dc.description.filFil: Jimenez, Veronica. California State University Fullerton. College of Natural Sciences and Mathematics. Center for Applied Biotechnology Studies. Department of Biological Science; USA.
dc.description.filFil: Tolmasky, Marcelo E. California State University Fullerton. College of Natural Sciences and Mathematics. Center for Applied Biotechnology Studies. Department of Biological Science; USA.
dc.description.filFil: Becka, Scott A. Louis Stokes Cleveland. Research Service and GRECC. Department of Veterans Afairs Medical Center; USA.
dc.description.filFil: Papp Wallace, Krisztina M. Louis Stokes Cleveland. Research Service and GRECC. Department of Veterans Afairs Medical Center; USA.
dc.description.filFil: Papp Wallace, Krisztina M. Case Western Reserve University School of Medicine. Departments of Medicine, Pharmacology, Molecular Biology and Microbiology, Biochemistry, Proteomics and Bioinformatics; USA.
dc.description.filFil: Papp Wallace, Krisztina M. CWRU-Cleveland VAMC Center for Antimicrobial Resistance and Epidemiology; USA.
dc.description.filFil: Bonomo, Robert A. Louis Stokes Cleveland. Research Service and GRECC. Department of Veterans Afairs Medical Center; USA.
dc.description.filFil: Bonomo, Robert A. Case Western Reserve University School of Medicine. Departments of Medicine, Pharmacology, Molecular Biology and Microbiology, Biochemistry, Proteomics and Bioinformatics; USA.
dc.description.filFil: Bonomo, Robert A. CWRU-Cleveland VAMC Center for Antimicrobial Resistance and Epidemiology; USA.
dc.description.filFil: Soler Bistue, Alfonso. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas (IIB-CONICET); Argentina.
dc.description.filFil: Sieira, Rodrigo. Fundación Instituto Leloir (IIBBA-CONICET); Argentina.
dc.description.filFil: Ramírez, María Soledad. California State University Fullerton. College of Natural Sciences and Mathematics. Center for Applied Biotechnology Studies. Department of Biological Science; USA.
dc.description.sponsorshipCleveland Department of Veterans Afairs: 1I01BX001974
dc.description.sponsorshipBiomedical Laboratory Research & Development Service: 1I01 BX002872
dc.description.sponsorshipGeriatric Research Education and Clinical Center
dc.format.extent1-13
dc.identifier.citationMartinez, Jasmine; Razo-Gutierrez, Chelsea; Le, Casin; Courville, Robert; Pimentel, Camila; et al.; Cerebrospinal fluid (CSF) augments metabolism and virulence expression factors in Acinetobacter baumannii; Nature Research; Scientific Reports; 11; 1; 2-2021; 1-13
dc.identifier.issn2045-2322
dc.identifier.urihttps://hdl.handle.net/2133/28417
dc.language.isoen
dc.publisherNature Research
dc.relation.publisherversionhttps://doi.org/10.1038/s41598-021-81714-6
dc.relation.publisherversionhttps://www.nature.com/articles/s41598-021-81714-6
dc.rightsopenAccess
dc.rights.holderMartínez, Jasmine
dc.rights.holderRazo Gutiérrez, Chelsea
dc.rights.holderLe, Casin
dc.rights.holderCourville, Robert
dc.rights.holderPimentel, Camila
dc.rights.holderLiu, Christine
dc.rights.holderFung, Sammie E.
dc.rights.holderTuttobene, Marisel Romina
dc.rights.holderPhan, Kimberly
dc.rights.holderVila, Alejandro J.
dc.rights.holderShahrestani, Parvin
dc.rights.holderJimenez, Veronica
dc.rights.holderTolmasky, Marcelo E.
dc.rights.holderBecka, Scott A.
dc.rights.holderPapp Wallace, Krisztina M.
dc.rights.holderBonomo, Robert A.
dc.rights.holderSoler Bistue, Alfonso
dc.rights.holderSieira, Rodrigo
dc.rights.holderRamírez, María Soledad
dc.rights.holderUniversidad Nacional de Rosario
dc.rights.textAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectAcinetobacter baumannii
dc.subjectDrug resistance
dc.subjectCerebrospinal fluid
dc.subjectVirulence
dc.titleCerebrospinal fuid (CSF) augments metabolism and virulence expression factors in Acinetobacter baumannii
dc.typearticulo
dc.type.collectionarticulo
dc.type.versionpublishedVersion

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