3-methylcrotonyl Coenzyme A (CoA) carboxylase complex is involved in the Xanthomonas citri subsp. citri lifestyle during citrus infection

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dc.citation.titlePLoS ONEes
dc.citation.volume13(6)es
dc.creatorTomassetti, Mauro
dc.creatorGaravaglia, Betiana Soledad
dc.creatorVranych, Cecilia Verónica
dc.creatorGottig, Natalia
dc.creatorOttado, Jorgelina
dc.creatorGramajo, Hugo Cesar
dc.creatorDiacovich, Lautaro
dc.date.accessioned2020-12-22T19:31:47Z
dc.date.available2020-12-22T19:31:47Z
dc.date.issued2018-06-07
dc.descriptionCitrus canker is a disease caused by the phytopathogen Xanthomonas citri subsp. citri (Xcc), bacterium which is unable to survive out of the host for extended periods of time. Once established inside the plant, the pathogen must compete for resources and evade the defenses of the host cell. However, a number of aspects of Xcc metabolic and nutritional state, during the epiphytic stage and at different phases of infection, are poorly characterized. The 3-methylcrotonyl-CoA carboxylase complex (MCC) is an essential enzyme for the catabolism of the branched-chain amino acid leucine, which prevents the accumulation of toxic intermediaries, facilitates the generation of branched chain fatty acids and/or provides energy to the cell. The MCC complexes belong to a group of acyl-CoA carboxylases (ACCase) enzymes dependent of biotin. In this work, we have identified two ORFs (XAC0263 and XAC0264) encoding for the α and β subunits of an acyl-CoA carboxylase complex from Xanthomonas and demonstrated that this enzyme has MCC activity both in vitro and in vivo. We also found that this MCC complex is conserved in a group of pathogenic gram negative bacteria. The generation and analysis of an Xcc mutant strain deficient in MCC showed less canker lesions in the interaction with the host plant, suggesting that the expression of these proteins is necessary for Xcc fitness during infection.es
dc.descriptionPara citar este articulo: Tomassetti M, Garavaglia BS, Vranych CV, Gottig N, Ottado J, Gramajo H, et al. (2018) 3- methylcrotonyl Coenzyme A (CoA) carboxylase complex is involved in the Xanthomonas citri subsp. citri lifestyle during citrus infection. PLoS ONE 13(6): e0198414. https://doi.org/10.1371/ journal.pone.0198414
dc.description.filFil: Tomassetti, Mauro. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.es
dc.description.filFil: Garavaglia, Betiana Soledad. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.es
dc.description.filFil: Vranych, Cecilia Verónica. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.es
dc.description.filFil: Gottig, Natalia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.es
dc.description.filFil: Ottado, Jorgelina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.es
dc.description.filFil: Gramajo, Hugo Cesar. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.es
dc.description.filFil: Diacovich, Lautaro. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.es
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET): PIP-2014-0764es
dc.description.sponsorshipAgencia Nacional de Promoción Científica y Tecnológica (ANPCyT): P. BID PICT 2014-1454 y PICT 2013-0625es
dc.formatapplication/pdf
dc.format.extent1-23es
dc.identifier.issn1932-6203es
dc.identifier.urihttp://hdl.handle.net/2133/19538
dc.language.isoenges
dc.publisherPublic Library of Science (PLOS)es
dc.relation.publisherversionhttps://doi.org/10.1371/journal.pone.0198414es
dc.relation.publisherversionhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0198414es
dc.rightsopenAccesses
dc.rights.holderUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticases
dc.rights.holderTomassetti, Mauroes
dc.rights.holderGaravaglia, Betiana Soledades
dc.rights.holderVranych, Cecilia Verónicaes
dc.rights.holderGottig, Nataliaes
dc.rights.holderOttado, Jorgelinaes
dc.rights.holderGramajo, Hugo Cesares
dc.rights.holderDiacovich, Lautaroes
dc.rights.textAttribution 4.0 International (CC BY 4.0)es
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subjectXanthomonas citri subsp. citries
dc.subjectCitrus Cankeres
dc.subjectMethylcrotonyl Coenzyme A (CoA) carboxylase complexes
dc.title3-methylcrotonyl Coenzyme A (CoA) carboxylase complex is involved in the Xanthomonas citri subsp. citri lifestyle during citrus infectiones
dc.typearticle
dc.typeartículo
dc.typepublishedVersion
dc.type.collectionarticulo
dc.type.versionpublishedVersiones

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