Endocannabinoids in Caenorhabditis elegans are essential for the mobilization of cholesterol from internal reserves

Vista sencilla de ítem
dc.citation.titleScientific Reportses
dc.citation.volume8es
dc.creatorGalles, Celina
dc.creatorPrez, Gastón M.
dc.creatorPenkov, Sider
dc.creatorBoland, Sebastian
dc.creatorPorta, Exequiel Oscar Jesús
dc.creatorAltabe, Silvia Graciela
dc.creatorLabadie, Guillermo Roberto
dc.creatorSchmidt, Ulrike
dc.creatorKnölker, Hans-Joachim
dc.creatorKurzchalia, Teymuras V.
dc.creatorDe Mendoza, Diego
dc.date.accessioned2021-02-19T00:24:59Z
dc.date.available2021-02-19T00:24:59Z
dc.date.issued2018-04-23
dc.descriptionProper cholesterol transport is crucial for the functionality of cells. In C. elegans, certain cholesterol derivatives called dafachronic acids (DAs) govern the entry into diapause. In their absence, worms form a developmentally arrested dauer larva. Thus, cholesterol transport to appropriate places for DA biosynthesis warrants the reproductive growth. Recently, we discovered a novel class of glycosphingolipids, PEGCs, required for cholesterol mobilization/transport from internal storage pools. Here, we identify other components involved in this process. We found that strains lacking polyunsaturated fatty acids (PUFAs) undergo increased dauer arrest when grown without cholesterol. This correlates with the depletion of the PUFA-derived endocannabinoids 2-arachidonoyl glycerol and anandamide. Feeding of these endocannabinoids inhibits dauer formation caused by PUFAs defciency or impaired cholesterol trafcking (e.g. in Niemann-Pick C1 or DAF-7/TGF-β mutants). Moreover, in parallel to PEGCs, endocannabinoids abolish the arrest induced by cholesterol depletion. These fndings reveal an unsuspected function of endocannabinoids in cholesterol trafcking regulation.es
dc.description.filFil: Galles, Celina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET). Laboratorio de Fisiología Microbiana; Argentina.es
dc.description.filFil: Prez, Gastón M. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET). Laboratorio de Fisiología Microbiana; Argentina.es
dc.description.filFil: Penkov, Sider. Max Planck Institute of Molecular Cell Biology and Genetics; Germany.es
dc.description.filFil: Boland, Sebastian. Harvard University. Harvard T.H. Chan School of Public Health. Department of Genetics and Complex Diseases; United States.es
dc.description.filFil: Boland, Sebastian. Harvard University. Harvard Medical School. Department of Cell Biology; United States.es
dc.description.filFil: Porta, Exequiel O. J. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Química Rosario (IQUIR -CONICET); Argentina.es
dc.description.filFil: Altabe, Silvia Graciela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET). Laboratorio de Fisiología Microbiana; Argentina.es
dc.description.filFil: Labadie, Guillermo Roberto. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Química Rosario (IQUIR -CONICET); Argentina.es
dc.description.filFil: Schmidt, Ulrike. Technische Universität Dresden. Department Chemie; Germany.es
dc.description.filFil: Knölker, Hans-Joachim. Technische Universität Dresden. Department Chemie; Germany.es
dc.description.filFil: Kurzchalia, Teymuras V. Max Planck Institute of Molecular Cell Biology and Genetics; Germany.es
dc.description.filFil: De Mendoza, Diego. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET). Laboratorio de Fisiología Microbiana; Argentina.es
dc.description.sponsorshipAgencia Nacional de Promoción Científca y Técnica (ANPCYT): PICT 2155 y PICT 3693es
dc.description.sponsorshipAlexander von Humboldt Foundationes
dc.description.sponsorshipFundación Bunge y Born- Instituto Max Planck.es
dc.formatapplication/pdf
dc.format.extent1-12es
dc.identifier.issn2045-2322es
dc.identifier.urihttp://hdl.handle.net/2133/19648
dc.language.isoenges
dc.publisherNaturees
dc.relation.publisherversionhttps://www.nature.com/articles/s41598-018-24925-8es
dc.relation.publisherversionhttps://doi.org/10.1038/s41598-018-24925-8es
dc.rightsopenAccesses
dc.rights.holderUniversidad Nacional de Rosarioes
dc.rights.holderGalles, Celinaes
dc.rights.holderPrez, Gastón M.es
dc.rights.holderPenkov, Sideres
dc.rights.holderBoland, Sebastianes
dc.rights.holderPorta, Exequiel O. J.es
dc.rights.holderLabadie, Guillermo Robertoes
dc.rights.holderSchmidt, Ulrikees
dc.rights.holderKnölker, Hans-Joachimes
dc.rights.holderKurzchalia, Teymuras V.es
dc.rights.holderDe Mendoza, Diegoes
dc.rights.textAttribution 4.0 International (CC BY 4.0)es
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subjectEndocannabinoidses
dc.subjectCaenorhabditis eleganses
dc.subjectCholesterol Transportes
dc.subjectGlycosphingolipidses
dc.titleEndocannabinoids in Caenorhabditis elegans are essential for the mobilization of cholesterol from internal reserveses
dc.typearticle
dc.typeartículo
dc.typepublishedVersion
dc.type.collectionarticulo
dc.type.versionpublishedVersiones

Archivos

Bloque original
Mostrando 1 - 1 de 1
Miniatura
Nombre:
188.pdf
Tamaño:
1.89 MB
Formato:
Adobe Portable Document Format
Descripción:
versión post-print
Descargar
Bloque de licencias
Mostrando 1 - 1 de 1
Nombre:
license.txt
Tamaño:
3.59 KB
Formato:
Item-specific license agreed upon to submission
Descripción:
Descargar

Colecciones

(FBIOyF) Departamento de Química Orgánica - Artículos