Hemocompatibility and hemorheological activity of aqueous extracts from Phyllanthus sellowianus: effects on in vitro glycated erythrocytes

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dc.citation.titleJournal of Herbal Medicine
dc.creatorMascaro Grosso, Hermano
dc.creatorBuszniez , Patricia
dc.creatorRiquelme, Bibiana Doris
dc.date.accessioned2024-11-22T17:39:21Z
dc.date.available2024-11-22T17:39:21Z
dc.date.issued2024-12
dc.description.abstractIntroduction: Phyllanthus sellowianus (Klotzsch) Müll Arg. is an Argentinian and Brazilian native plant used to treat diabetes. In vascular diseases like diabetes, erythrocytes form anomalous aggregates resistant to dissociation, associated with high glucose levels. This work aims to evaluate the hemocompatibility and hemorheological activity of aqueous extracts from P. sellowianus and its anti-diabetic activity using in vitro glycated erythrocytes as occur by hyperglycemia. Methods: For this work, P. sellowianus specimens were collected on the Nogoyá River coast, Argentina, and morphoanatomical identified. Infusion, maceration, digestion, and cooking extracts were prepared with collected leaves and bark using saline as solvent. The osmolality and pH of the extracts were adjusted to an adequate interaction with human cells. Red blood cells (RBCs) from healthy donors (n=3) were used and incubated with glucose solution for in vitro simulation of the hyperglycemia effects. RBCs were incubated with the aqueous extracts to assess their hemocompatibility. Also, glycated RBCs were incubated with the extracts to analyze their anti-diabetic properties. Erythrocyte Rheometer and Optical Chip Aggregameter were used for the hemorheological evaluation. Results: Assays with the different P. sellowianus extracts modified the viscoelasticity and aggregation of RBCs. Moreover, in some cases, P. sellowianus extracts reversed the in vitro glycation effect and hemorheological parameters got nearer control values. Conclusion: These results would be helpful for the development of pharmaceutical formulations using this species or its phytocomponents. They also give relevant information to understand the action mechanisms through which these extracts or their components can be used as anti-diabetics in Phytomedicine.
dc.description.filFil: Mascaro Grosso, Hermano. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina.
dc.description.filFil: Buszniez, Patricia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina.
dc.description.filFil: Riquelme, Bibiana Doris. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Física Rosario (IFIR-CONICET); Argentina.
dc.description.filFil: Riquelme, Bibiana Doris. Universidad Nacional de Rosario. Consejo de Investigaciones de la UNR (CIUNR); Argentina.
dc.description.sponsorshipUniversidad Nacional de Rosario (UNR): Project BIO593
dc.description.versionpeerreviewed
dc.format.extent100945
dc.identifier.issn2210-8033
dc.identifier.urihttps://hdl.handle.net/2133/28189
dc.language.isoen
dc.publisherElsevier
dc.relation.publisherversionhttps://doi.org/10.1016/j.hermed.2024.100945
dc.rightsembargoedAccess
dc.rights.holderUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas
dc.rights.holderElsevier
dc.rights.holderMascaro Grosso, Hermano
dc.rights.holderBuszniez, Patricia
dc.rights.holderRiquelme, Bibiana Doris
dc.rights.textAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectPhyllanthus sellowianus
dc.subjectDiabetes
dc.subjectErythrocyte aggregation
dc.subjectErythrocyte viscoelasticity
dc.subjectHemorheology
dc.titleHemocompatibility and hemorheological activity of aqueous extracts from Phyllanthus sellowianus: effects on in vitro glycated erythrocytes
dc.typearticulo
dc.type.collectionarticulo
dc.type.versionacceptedVersion

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(FBIOyF) Departamento de Química Física - Artículo de Revista