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Cholesterol can modulate mitochondrial aquaporin-8 expression in human hepatic cells

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dc.citation.titleIUBMB Life
dc.citation.volume69
dc.creatorDanielli, Mauro
dc.creatorCapiglioni, Alejo M.
dc.creatorMarrone, Julieta
dc.creatorCalamita, Giuseppe
dc.creatorMarinelli, Raúl A.
dc.date.accessioned2024-11-05T14:14:06Z
dc.date.available2024-11-05T14:14:06Z
dc.date.issued2017-03-20
dc.description.abstractHepatocyte mitochondrial aquaporin-8 (mtAQP8) works as a multifunctional membrane channel protein that facilitates the uptake of ammonia for its detoxification to urea as well as the mitochondrial release of hydrogen peroxide. Since early oligonucleotide microarray studies in liver of cholesterol-fed mice showed an AQP8 downregulation, we tested whether alterations of cholesterol content per se modulate mtAQP8 expression in human hepatocyte-derived Huh-7 cells. Cholesterol loading with methyl-β-cyclodextrin (mβCD):cholesterol complexes downregulated the proteolytic activation of cholesterol-responsive sterol regulatory element-binding protein (SREBP) transcriptions factors 1 and 2, and the expression of the target gene 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR). Under such conditions, mtAQP8 mRNA and protein expressions were significantly reduced. In contrast, cholesterol depletion using mβCD alone increased SREBP-1 and 2 activation and upregulated HMGCR and mtAQP8 mRNA and protein expressions. The results suggest that cholesterol can regulate transcriptionally human hepatocyte mtAQP8 expression likely via SREBPs. The functional implications of our findings are discussed.
dc.description.filFil: Danielli, Mauro. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Fisiología Experimental (IFISE-CONICET); Argentina.
dc.description.filFil: Capiglioni, Alejo M. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Fisiología Experimental (IFISE-CONICET); Argentina.
dc.description.filFil: Marrone, Julieta. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Fisiología Experimental (IFISE-CONICET); Argentina.
dc.description.filFil: Calamita, Giuseppe. Università degli Studi di Bari. Dipartimento di Bioscienze, Biotecnologie e Biofarmaceutica; Italia.
dc.description.filFil: Marinelli, Raúl A. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Fisiología Experimental (IFISE-CONICET); Argentina.
dc.description.sponsorshipAgencia Nacional de Promoción de la Investigación, el Desarrollo Tecnológico y la Innovación (Agencia I+D+i): Grant PICT 1217
dc.description.versionpeerreviewed
dc.format.extent1-6
dc.identifier.citationDanielli, M., Capiglioni, A.M., Marrone, J., Calamita, G. and Marinelli, R.A. (2017). Cholesterol can modulate mitochondrial aquaporin-8 expression in human hepatic cells. IUBMB Life, 69(5), 341-346. https://doi.org/10.1002/iub.1615
dc.identifier.e-issn1521-6551
dc.identifier.issn1521-6543
dc.identifier.urihttps://hdl.handle.net/2133/28047
dc.language.isoen
dc.publisherWiley
dc.relation.publisherversionhttps://doi.org/10.1002/iub.1615
dc.relation.publisherversionhttps://iubmb.onlinelibrary.wiley.com/doi/10.1002/iub.1615
dc.rightsopenAccess
dc.rights.holderDanielli, Mauro
dc.rights.holderCapiglioni, Alejo M.
dc.rights.holderMarrone, Julieta
dc.rights.holderCalamita, Giuseppe
dc.rights.holderMarinelli, Raúl A.
dc.rights.holderUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas
dc.rights.holderInternational Union of Biochemistry and Molecular Biology
dc.rights.textAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectCholesterol
dc.subjectMitochondrial aquaporin-8
dc.subjectHepatic cells
dc.subjectAquaporins
dc.subjectHepatocytes
dc.titleCholesterol can modulate mitochondrial aquaporin-8 expression in human hepatic cells
dc.typearticulo
dc.type.collectionarticulo
dc.type.versionpublishedVersion

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