Mycobacteriophage CRB2 defines a new subcluster in mycobacteriophage classification

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dc.citation.titlePLoS ONE 14(2)es
dc.creatorSuarez, Cristian Alejandro
dc.creatorFranceschelli, Jorgelina Judith
dc.creatorMorbidoni, Héctor Ricardo
dc.date.accessioned2019-03-14T20:11:36Z
dc.date.available2019-03-14T20:11:36Z
dc.date.issued2019-02-27
dc.descriptionMycobacteriophages are viruses -mostly temperates- that infect Mycobacterium smegmatis and sometimes Mycobacterium tuberculosis. Mycobacteriophages are grouped in clusters on the basis of the overall nucleotide sequence homology, being further divided in subclusters as more mycobacteriophage genomes are sequenced and annotated. As part of our on-going screening for novel isolates, we herein report the bioinformatics analysis of CRB2, a mycobacteriophage belonging into the Siphoviridae family that propagates at 30°C. CRB2 has a 72,217 bp genome with a 69.78% GC content that belongs to Cluster B; nucleotide comparison with other B cluster members positions CRB2 as the sole member of a new subcluster, B9, being mycobacteriophage Saguaro (belonging into subcluster B7) its closest relative. Sequencing and annotation of 14 mycobacteriophages isolated by our group has yielded six cluster A members, a singleton, four of the five members of subcluster B6, one of the three reported members of subcluster G4, and CRB2 which defines subcluster B9. Considering the massive mycobacteriophage search performed in USA and the relatively rarity of our phages, we propose that factors other than size of the sampling determine the variability of mycobacteriophage distribution, and thus a world-wide concerted mining would most likely bring extremely rare and yet undiscovered mycobacteriophages.es
dc.description.filFil: Suarez, Cristian Alejandro. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Microbiología Molecular; Argentinaes
dc.description.filFil: Franceschelli, Jorgelina Judith. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Microbiología Molecular; Argentinaes
dc.description.filFil: Morbidoni, Héctor Ricardo. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Microbiología Molecular; Argentinaes
dc.description.sponsorship(PICT 02337 and PICT 1406 to HRM) from the National Agency for Science and Technology Promotion (ANPCyT), Argentina. HRM is a career member of Consejo de Investigaciones de la Universidad Nacional de Rosario (CIUNR), CAS and JJF are careerr member and post-doctoral fellow of Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.es
dc.formatapplication/pdf
dc.format.extent1-19es
dc.identifier.issn1932-6203es
dc.identifier.urihttp://hdl.handle.net/2133/14224
dc.language.isoenges
dc.publisherPLOSes
dc.relation.publisherversionhttps://doi.org/10.1371/journal.pone.0212365es
dc.rightsopenAccesses
dc.rights.holderSuarez et al.es
dc.rights.texthttps://creativecommons.org/licenses/by/4.0/es
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subjectBacteriophageses
dc.subjectGenome analysises
dc.subjectComparative genomicses
dc.subjectViral genomicses
dc.subjectGenomic databaseses
dc.subjectSequence alignmentes
dc.subjectSequence databaseses
dc.titleMycobacteriophage CRB2 defines a new subcluster in mycobacteriophage classificationes
dc.typearticle
dc.typeartículo
dc.typepublishedVersion
dc.type.collectionarticulo
dc.type.versionpublishedVersiones

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