Relationship between genotoxic effects of breast cancer treatments and patient basal DNA integrity

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dc.creator Capitaine Funes, Juan
dc.creator Massa, Estefania
dc.creator Cipulli, Germán
dc.creator Benitez Gil, Alfonso
dc.creator Capitaine Funes, Carlos
dc.creator Tozzini, Roberto
dc.creator Ghersevich, Sergio
dc.creator Ceballos, María Paula
dc.date.accessioned 2017-11-23T18:41:19Z
dc.date.available 2017-11-23T18:41:19Z
dc.date.issued 2014-04-15
dc.identifier.issn 0731-8898 es
dc.identifier.uri http://hdl.handle.net/2133/9378
dc.description It is accepted that radiotherapy and chemotherapy cause genotoxic effects as part of their therapy side effects, which are highly variable among different patients. This study evaluated DNA integrity using the comet assay in peripheral blood lymphocytes from breast cancer patients before (“pre-treatment patients”; n = 47) and after (“post-treatment patients”; n = 24) radiotherapy and/or chemotherapy treatment and from healthy donors (n = 15). Comet evaluation was made by visual (types 0-4) and digital (percentage of DNA remaining in the comet head = % head DNA) analysis. The association between the level of DNA damage and cancer prognostic factors was assessed. The treatments caused a significant increase in DNA damage registered by both visual (p < 0.001) and digital (p < 0.001) analysis. No significant associations between the level of DNA damage in pre-treatment patients and cancer prognostic factors were found. Results showed a significant correlation between the comet results from each patient before and after treatment (r = 0.64, p = 0.001). The % head DNA in post-treatment samples from patients with high level of DNA damage before treatment (30.3 ± 3.1%, p < 0.01) was lower than in post-treatment samples from patient with low to medium level of DNA damage before therapy (49.2 ± 4.4%). Results support the usefulness of the comet assay as a sensitive technique to evaluate the basal DNA status and the damage caused by cancer treatments. The assay could contribute to the treatment decisions, taking into account the patients’ basal DNA damage before therapy. es
dc.format application/pdf
dc.format.extent 111-121 es
dc.language.iso eng es
dc.publisher BEGELL HOUSE INC es
dc.rights openAccess es
dc.subject Ensayo cometa es
dc.subject Daño genotóxico es
dc.subject Cáncer mamas es
dc.subject Factor pronóstico es
dc.subject Peripheral blood lymphocytes es
dc.subject Comet assay es
dc.subject Genotoxic damage es
dc.title Relationship between genotoxic effects of breast cancer treatments and patient basal DNA integrity es
dc.type article
dc.type artículo
dc.type acceptedVersion
dc.rights.holder Begell House Inc. es
dc.rights.holder Ceballos, María Paula es
dc.rights.holder Capitaine Funes, Juan es
dc.rights.holder Massa, Estefanía es
dc.rights.holder Cipulli, Germán es
dc.rights.holder Benitez Gil, Alfonso es
dc.rights.holder Capitaine Funes, Carlos es
dc.rights.holder Tozzini, Roberto es
dc.rights.holder Ghersevich, Sergio es
dc.relation.publisherversion http://www.dl.begellhouse.com/journals/0ff459a57a4c08d0,249005dc454eb8de,570be1034750e71e.html es
dc.relation.publisherversion DOI: 10.1615/JEnvironPatholToxicolOncol.2014010592 es
dc.citation.title Journal of Environmental Pathology, Toxicology and Oncology es
dc.citation.volume 33(2) es
dc.description.fil Fil: Capitaine Funes, Juan. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentina es
dc.description.fil Fil: Ceballos, María Paula. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Fisiología Experimental (UNR-CONICET); Argentina es
dc.description.fil Fil: Capitaine Funes, Juan. Provincia de Santa Fe. Hospital Provincial del Centenario. Servicio de Mastología; Argentina es
dc.description.fil Fil: Massa, Estefania. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina es
dc.description.fil Fil: Cipulli, Germán. Provincia de Santa Fe. Hospital Provincial del Centenario. Servicio de Mastología; Argentina es
dc.description.fil Fil: Benitez Gil, Alfonso. Provincia de Santa Fe. Hospital Provincial. Servicio de Mastología; Argentina es
dc.description.fil Fil: Capitaine Funes, Carlos. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentina es
dc.description.fil Fil: Capitaine Funes, Carlos. Provincia de Santa Fe. Hospital Provincial del Centenario. Servicio de Mastología; Argentina es
dc.description.fil Fil: Tozzini, Roberto. Universidad Nacional de Rosario. Facultad de Ciencias Médicas; Argentina es
dc.description.fil Fil: Tozzini, Roberto. Provincia de Santa Fe. Hospital Provincial del Centenario. Servicio de Mastología; Argentina es
dc.description.fil Fil: Ghersevich, Sergio. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina es
dc.type.collection articulo
dc.type.version acceptedVersion es


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