On-line generation of third-order liquid chromatography–excitation-emission fluorescence matrix data. Quantitation of heavy-polycyclic aromatic hydrocarbons

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dc.creator Carabajal, Maira Daniela
dc.creator Arancibia, Juan Alberto
dc.creator Escandar, Graciela Mónica
dc.date.accessioned 2019-02-08T21:27:33Z
dc.date.available 2019-02-08T21:27:33Z
dc.date.issued 2017-12-08
dc.identifier.issn 0021-9673
dc.identifier.uri http://hdl.handle.net/2133/13923
dc.description For the first time, third-order liquid chromatography with excitation-emission fluorescence matrix detection (LC-EEFM) data were generated on-line and chemometrically processed for the simultaneous quantitation of the heavy-polycyclic aromatic hydrocarbons fluoranthene, pyrene, benz[a]anthracene, chrysene, benzo[b]fluoranthene, benzo[k]fluoranthene, benzo[a]pyrene, and dibenz[a,h]anthracene. The applied experimental strategy is very simple, and is based on the reduction of the linear flow rate by fitting a larger diameter connecting-tube between the column outlet and the fluorimetric detector. In this way, EEFMs were successfully recorded on-line, without involving a large total analysis time. Because in the studied system quadrilinearity was fulfilled, four-way parallel factor (PARAFAC) analysis was applied for data processing. The second-order advantage, which is an intrinsic property of data of at least second-order, allowed the quantification of the analytes in interfering media. Moreover, resolution of the system with a high degree of collinearity was achieved thanks to the third-order advantage. In addition to a selectivity improvement, third-order/four-way calibration increased the sensitivity, with limits of detection in the range of 0.4 − 2.9 ng mL−1. After a solid-phase extraction procedure with C18 membranes, considerably lower concentrations (between 0.033–2.70 ng mL−1) were determined in real waters, with most recoveries in the range 90–106%. es
dc.description.sponsorship Universidad Nacional de Rosario (Argentina): Proyecto BIO 237 es
dc.description.sponsorship Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET): Proyecto PIP 0163 es
dc.description.sponsorship Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT): PICT 2016-1122 es
dc.format application/pdf
dc.format.extent 61-69
dc.language.iso eng es
dc.publisher Elsevier es
dc.rights embargoedAccess es
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es *
dc.subject Liquid chromatography-excitation-emission fluorescence es
dc.subject Third-order/four-way calibration es
dc.subject Third-order advantage es
dc.subject Heavy-polycyclic aromatic hydrocarbons es
dc.title On-line generation of third-order liquid chromatography–excitation-emission fluorescence matrix data. Quantitation of heavy-polycyclic aromatic hydrocarbons es
dc.rights.holder Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas es
dc.rights.holder Elsevier es
dc.rights.holder Carabajal, Maira Daniela es
dc.rights.holder Arancibia, Juan Alberto es
dc.rights.holder Escandar, Graciela Mónica es
dc.relation.publisherversion http://dx.doi.org/10.1016/j.chroma.2017.10.057 es
dc.relation.publisherversion https://www.sciencedirect.com/science/article/pii/S0021967317315637?via%3Dihub#ack0005 es
dc.rights.text Atribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0) es
dc.citation.title Journal of Chromatography A en
dc.citation.volume 1527
dc.description.fil Fil: Carabajal, Maira Daniela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Química Rosario (IQUIR-CONICET); Argentina. es
dc.description.fil Fil: Arancibia, Juan Alberto. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Química Rosario (IQUIR-CONICET); Argentina. es
dc.description.fil Fil: Escandar, Graciela Mónica. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Química Rosario (IQUIR-CONICET); Argentina. es


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