Examinando por Autor "Villalba, Juan Manuel"

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  • Miniatura
    ÍtemAcceso Abierto
    A transmembrane Histidine Kinase functions as a pH sensor
    (MDPI, 2020-08-14) Bortolotti, Ana; Vazquez, Daniela Belén; Almada, Juan Cruz; Inda, María Eugenia; Drusin, Salvador Iván; Villalba, Juan Manuel; Moreno, Diego M.; Ruysschaert, Jean Marie; Cybulski, Larisa Estefanía
  • Miniatura
    ÍtemAcceso Abierto
    Culturing bacteria from Caenorhabditis elegans gut to assess colonization proficiency
    (Bio-protocol L.L.C., 2017-06-20) Rodríguez Ayala, Facundo; Cogliati, Sebastián; Bauman, Carlos; Leñini, Cecilia; Bartolini, Marco; Villalba, Juan Manuel; Argañaraz, Federico; Grau, Roberto Ricardo
    Determining an accurate count of intestinal bacteria from Caenorhabditis elegans is one critical way to assess colonization proficiency by a given bacteria. This can be accomplished by culturing appropriate dilutions of worm gut bacteria on selective or differential agarized media. Because of the high concentration of bacteria in gut worm, dilution is necessary before plating onto growth media. Serial dilutions can reduce the concentration of the original intestinal sample to levels low enough for single colonies to be grown on media plates, allowing for the calculation of the initial counts of bacteria in the intestinal sample.