Examinando por Autor "Racca, Liliana"

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    ÍtemAcceso Abierto
    Acute diarrhoea in children: determination of duration using a combined bismuth hydroxide gel and oral rehydration solution therapy vs. oral rehydration solution
    (MDPI, 2016-12-21) Oviedo, Adriana; Díaz, Mirna; Valenzuela, María Laura; Vidal, Victoria; Racca, Liliana; Bottai, Hebe; Priore, Graciela; Peluffo, Graciela; Di Bartolomeo, Susana; Cabral, Graciela; Toca, María del Carmen
    Oral rehydration salt (ORS) treatment in young children with acute diarrhoea (AD) has contributed to decrease mortality associated with dehydration although effective strategies to reduce morbidity associated with this disease are required. The aim of this study was to evaluate the diarrhoea duration when using combined colloidal bismuth hydroxide gel (CBHG) and oral rehydration salt treatment compared with ORS therapy in children with AD. We designed a double-blind, randomised prospective study with treatment and control groups. Patients aged one to 12 years, with no prior pathology and with AD of less than 48 h were included. The Chi-squared and Mann-Whitney tests were used, as well as the Cox proportional hazards model and the Kaplan-Meier estimator. Patients were randomised into an ORS and CBHG treatment group and a control group for ORS plus placebo. (Average age: 3.2 years). The result of the post-treatment evaluation with respect to the average duration of AD was 25.5 h for the treated group vs. 41.5 h for the control group (p = 0.015). The average number of stools was 4.8 in the treated group and 8.2 in the control group (p = 0.032). We conclude that the use of CBHG plus ORS significantly reduced the duration of AD, the number of stools and the percentage of children with persistent AD after 24 h of treatment compared to the control group. AD remitted almost twice as fast in patients treated with CBHG and ORS compared to those who received ORS plus placebo.
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    ÍtemAcceso Abierto
    Assessing endocrine and immune parameters in human immunodeficiency virus-infected patients before and after the immune reconstitution inflammatory syndrome
    (Brazilian Society of Endocrinology and Metabolism, 2018-02) Rateni, Liliana Beatriz; Lupo, Sergio; Racca, Liliana; Palazzi, Jorge; Ghersevich, Sergio Albino
    Objective: The present study compares immune and endocrine parameters between HIV-infected patients who underwent the Immune Reconstitution Inflammatory Syndrome (IRIS-P) during antiretroviral therapy (ART) and HIV-patients who did not undergo the syndrome (non-IRIS-P). Materials and methods: Blood samples were obtained from 31 HIV-infected patients (15 IRIS-P and 16 non-IRIS-P) before ART (BT) and 48 ± 2 weeks after treatment initiation (AT). Plasma Interleukin-6 (IL-6) and Interleukin-18 (IL-18) were determined by ELISA. Cortisol, dehydroepiandrosterone sulfate (DHEA-S) and thyroxin concentrations were measured using chemiluminescence immune methods. Results: Concentrations of IL-6 (7.9 ± 1.9 pg/mL) and IL-18 (951.5 ± 233.0 pg/mL) were significantly higher (p < 0.05) in IRIS-P than in non-IRIS-P (3.9 ± 1.0 pg/mL and 461.0 ± 84.4 pg/mL, respectively) BT. Mean T4 plasma level significantly decreased in both groups of patients after treatment (p < 0.05). In both groups cortisol levels were similar before and after ART (p > 0.05). Levels of DHEA-S in IRIS-P decreased AT (1080.5 ± 124.2 vs. 782.5 ± 123.8 ng/mL, p < 0.05) and they were significantly lower than in non-IRIS-P (782.5 ± 123.8 vs. 1203.7 ± 144.0 ng/mL, p < 0.05). IRIS-P showed higher values of IL-6 and IL-18 BT and lower levels of DHEA-S AT than in non-IRIS-P. Conclusion: These parameters could contribute to differentiate IRIS-P from non-IRIS-P. The significant decrease in DHEA-S levels in IRIS-P after ART might suggest a different adrenal response
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    Estudio taxonómico de aislamientos clínicos de Trichophyton en Rosario, Argentina
    (Elsevier, 2014-01-03) Tartabini, Mirta L.; Bonino, Guillermo S.; Racca, Liliana; Luque, Alicia Graciela
    Debido al pleomorfaismo y la variabilidad cultural que presentan las especies del género Trichophyton, los métodos de identificación basados exclusivamente en caracteres morfológicos a menudo no son suficientes para su clasificación. El objetivo de este estudio fue probar un conjunto de métodos fenotípicos para identificar aislamientos fúngicos pertenecientes al género Trichophyton empleando una técnica de taxonomía molecular como método de referencia. Se utilizaron 56 aislamientos clínicos y 6 cepas de referencia, con los que se realizaron estudios macro y micromorfológicos, así como las siguientes pruebas bioquímicas y fisiológicas: perforación del pelo in vitro, requerimientos nutricionales en medios agar Trichophyton, producción de ureasa y crecimiento en el medio agar púrpura de bromocresol-leche-glucosa (APBC-L-G). A su vez se realizó una PCR fingerprinting utilizando el cebador simple (GACA)4. Como resultado de la reacción de PCR se observaron perfiles específicos bien diferenciables para Microsporum canis, Epidermophyton floccosum, Trichophyton rubrum y Trichophyton interdigitale, pero un mismo perfil para Arthroderma benhamiae y Trichophyton erinacei. Del total de los aislamientos clínicos evaluados, 39 coincidieron con el perfil de T. rubrum, 13 con el de A. benhamiae y 4 con el de T. interdigitale. El ensayo fenotípico más útil para diferenciar T. rubrum del complejo T. mentagrophytes fue la alcalinización del medio APBC-L-G. Con las pruebas fenotípicas se pudo diferenciar T. rubrum de los aislamientos del complejo T. mentagrophytes, pero no las especies pertenecientes a dicho complejo. La técnica molecular permitió caracterizar tanto los aislamientos de T. rubrum como los pertenecientes al complejo T. mentagrophytes, que en nuestro estudio correspondieron a T. interdigitale y Trichophyton sp. anamorfo de A. benhamiae.
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    ÍtemAcceso Abierto
    Expression of the gene encoding secretor type galactoside 2 α fucosyltransferase (FUT2) and ABH antigens in patients with oral lesions
    (Sociedad Española de Medicina Oral, 2012) Campi, Carlos; Escovich, Livia; Moreno, Alejandra Soledad; Racca, Liliana; Cotorruelo, Carlos; Biondi, Claudia Silvia
    Objective: The aim of this work was to evaluate the expression of FUT2 gene in saliva and histo ABH antigens of patients with oral lesions. Study Design: In total 178 subjects were examined, half of whom suffered from oral pre-cancerous and cancerous lesions, while the other half were the healthy control group We analyzed the FUT 2 polymorphism by ASO-PCR (allele specific oligonucleotid – polymerase chain reaction) with specific primers for G428 allele and the wild type allele of FUT2 gene. To reveal A, B and H antigens in tissue sections of the patients (n= 89) we used a modified specific red cell adherence technique. Results: We found a high intensity of oral disease in the non-secretor group (OR = 2.43). A total of 58% of the patients with oral pre-cancerous and cancerous lesions was non secretors (se_/_), in contrast with the healthy population (21.5%). A strongly positive reaction was defined as a sheet of indicator erythrocytes adhered to the epithelial cells. In 31 of the 54 samples analyzed the test showed slightly positive results on atypical areas, and there was a complete antigen deletion in areas affected by neoplasia. Nineteen samples showed a total absence of ABH antigens in both histologically normal and pathological areas. Blood group antigens were expressed at a high level in benign and highly differentiated malignant tumors. In poorly differentiated malignant tumors, they were mostly absent. Conclusion: Considering these results we suggest the use of this method to monitor probable preneoplastic lesions in risk population, especially in those with no secretor status (absence of FUT2 gene).
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    Parasitosis intestinales en una población pediátrica de la ciudad de Rosario, Santa Fe, Argentina
    (Federación Bioquímica de la Provincia de Buenos Aires, 2011-04) Indelman, Paula; Echenique, Claudia G.; Bertorini, Griselda; Racca, Liliana; Gomez, Carlos; Luque, Alicia Graciela; Magaró, Hortensia María
    Las parasitosis intestinales afectan principalmente a los niños. El objetivo de este trabajo es conocer la situación actual en una población pediátrica de diferentes zonas de la ciudad de Rosario, Santa Fe (Argentina) y comparar la prevalencia parasitaria con estudios similares realizados en los períodos 1983/1984, 1990/1991, 1992/1993 y 2007/2008. Se recolectaron muestras de materia fecal por deposición espontánea de 112 pacientes, 51 niñas y 61 niños, con edades comprendidas entre 4 meses y 16 años, provenientes de 10 Centros de Atención Primaria de la Salud (Secretaría de Salud Pública - Municipalidad de Rosario). Las muestras fueron sometidas a examen macroscópico y microscópico directo y a métodos de concentración. En el período 2007/2008 los parásitos más hallados fueron Blastocystis hominis, Giardia lamblia y Ascaris lumbricoides. La prevalencia parasitaria disminuyó del 50% al 41%. Blastocystis hominis aumentó a través del tiempo; Giardia lamblia mantuvo valores constantes en las cuatro etapas; Entamoeba coli disminuyó en los últimos 14 años y Ascaris lumbricoides aumentó significativamente en relación con el período 1983/1984. Disminuyeron los individuos poliparasitados y aumentaron los monoparasitados con respecto a años anteriores. La disminución de las enteroparasitosis podría deberse a políticas de saneamiento ambiental, campañas de prevención y desparasitación realizadas desde los distintos efectores de salud municipales.