Examinando por Autor "Poljak, Mario"
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Ítem Acceso Abierto A preliminary study of the virome of the South American Free-Tailed Bats (Tadarida brasiliensis) and identification of two novel mammalian viruses(MDPI, 2020-04-09) Bolatti, Elisa María; Zorec, Tomaž M.; Montani, María E.; Hošnjak, Lea; Chouhy, Diego; Viarengo, Gastón; Casal, Pablo E.; Barquez, Rubén M.; Poljak, Mario; Giri, Adriana Angélica; Irene Villa, German Saigo, Mauricio Taborda, and Violeta Di Domenica, for collecting bat samplesÍtem Acceso Abierto Assessing Gammapapillomavirus infections of mucosal epithelia with two broad-spectrum PCR protocols(BMC, 2020-04-07) Bolatti, Elisa María; Hošnjak, Lea; Chouhy, Diego; Casal, Pablo E.; Re Louhau, María F.; Bottai, Hebe; Komloš , Kristina Fujs; Poljak, Mario; Giri, Adriana Angélica; https://orcid.org/0000-0003-4925-9075Background: Human papillomaviruses (HPVs) have been divided into mucosal and cutaneous types according to their primary epithelial tissue tropism. However, recent studies showed the presence of several cutaneous types in mucosal lesions and healthy mucosa from different anatomical sites. Methods: Here, the HPV prevalence and type-specific distribution were assessed in a variety of mucosal samples from 435 individuals using a combination of two established broad-spectrum primer systems: Gamma-PV PCR and CUT PCR. Results: Overall HPV prevalence in anal canal swabs, cervical cancer biopsies, genital warts and oral swabs was 85, 47, 62 and 4%, respectively. In anal canal swabs, Alpha-PVs were most frequently found (59%), followed by Gamma-(37%) and Beta-PVs (4%). The prevalence and persistence of HPV infection in the anal canal of 226 individuals were further explored. Overall HPV, Gamma-PVs and multiple HPV infections were significantly higher in men vs. women (p = 0.034, p = 0.027 and p = 0.003, respectively); multiple HPV infections were more common in individuals ≤40 years (p = 0.05), and significantly higher prevalence of Gamma-PVs and multiple HPV infections was observed in HIV-1-positive vs. HIV-1-negative individuals (p = 0.003 and p = 0.04, respectively). Out of 21 patients with follow-up anal swabs, only one persistent infection with the same type (HPV58) was detected. Conclusions: Our findings suggest that Gamma-PVs (except species Gamma-6) are ubiquitous viruses with dual muco-cutaneous tissue tropism. Anal canal Gamma-PV infections may be associated with sexual behavior and the host immune status. This study expands the knowledge on Gamma-PVs’ tissue tropism, providing valuable data on the characteristics of HPV infection in the anal canal.Ítem Acceso Abierto Genome announcement : complete genome sequence of a novel Mupapillomavirus, HPV204(Association of Slovenian Dermatovenerologists, 2015) Chouhy, Diego; Bolatti, Elisa María; Giri, Adriana Angélica; Poljak, Mario; Kocjan, Boštjan J.; Šterbenc, Anja; Hošnjak, LeaÍtem Acceso Abierto Identification and characterization of novel alphacoronaviruses in Tadarida brasiliensis (Chiroptera, Molossidae) from Argentina: insights into recombination as a mechanism favoring bat coronavirus cross-species transmission(ASM, 2023-09-11) Cerri, Agustina; Bolatti, Elisa María; Zorec, Tomaz M.; Montani, Maria E.; Rimondi, Agustina; Hosnjak, Lea; Casal, Pablo E.; Di Domenica, Violeta; Barquez, Rubén M.; Poljak, Mario; Giri, Adriana Angélica; https://orcid.org/0000-0003-4925-9075; https://orcid.org/0000-0001-6467-0650Bats are reservoirs of various coronaviruses that can jump between bat species or other mammalian hosts, including humans. This article explores coronavirus infection in three bat species (Tadarida brasiliensis, Eumops bonariensis, and Molossus molossus) of the family Molossidae from Argentina using whole viral metagenome analysis. Fecal samples of 47 bats from three semiurban or highly urbanized areas of the province of Santa Fe were investigated. After viral particle enrichment, total RNA was sequenced using the Illumina NextSeq 550 instrument; the reads were assembled into contigs and taxonomically and phylogenetically analyzed. Three novel complete Alphacoronavirus (AlphaCoV) genomes (Tb1–3) and two partial sequences were identified in T. brasiliensis (Tb4–5), and an additional four partial sequences were identified in M. molossus (Mm1–4). Phylogenomic analysis showed that the novel AlphaCoV clustered in two different lineages distinct from the 15 officially recognized AlphaCoV subgenera. Tb2 and Tb3 isolates appeared to be variants of the same virus, probably involved in a persistent infectious cycle within the T. brasiliensis colony. Using recombination analysis, we detected a statistically significant event in Spike gene, which was reinforced by phylogenetic tree incongruence analysis, involving novel Tb1 and AlphaCoVs identified in Eptesicus fuscus (family Vespertilionidae) from the U.S. The putative recombinant region is in the S1 subdomain of the Spike gene, encompassing the potential receptor-binding domain of AlphaCoVs. This study reports the firstfirstAlphaCoV genomes in molossids from the Americas and provides new insights into recombination as an important mode of evolution of coronaviruses involved in cross-species transmission.Ítem Acceso Abierto Molecular characterization, tissue tropism, and genetic variability of the novel Mupapillomavirus type HPV204 and phylogenetically related types HPV1 and HPV63.(PLOS, 2017) Chouhy, Diego; Bolatti, Elisa María; Seme, Katja; Poljak, Mario; Giri, Adriana Angélica; Šterbenc, Anja; Hošnjak, Lea; Oštrbenk, Anja; Kocjan, Boštjan J.; Luzar, BoštjanÍtem Acceso Abierto Viral Metagenomic Data Analyses of Five New World Bat Species from Argentina: Identification of 35 Novel DNA Viruses(MDPI, 2022-01-24) Bolatti, Elisa María; Viarengo, Gastón; Zorec, Tomaž M; Cerri, Agustina; Montani, María E.; Hošnjak, Lea; Casal, Pablo E.; Bortolotto, Eugenia; Di Domenica, Violeta; Chouhy, Diego; Allasia, María Belén; Barquez, Rubén M.; Poljak, Mario; Giri, Adriana Angélica