Examinando por Autor "Pierella Karlusich, Juan J."

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    Environmental selection pressures related to iron utilization are involved in the loss of the flavodoxin gene from the plant genome
    (Oxford University Press, 2015-02-16) Pierella Karlusich, Juan J.; Ceccoli, Romina Denis; Graña, Martín; Romero, Héctor; Carrillo, Néstor
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    Genome-wide plant responses during the non-host interaction of tobacco with the Hemibiotrophic Bacterium Xanthomonas campestris pv. Vesicatoria
    (Frontiers Media, 2017-07-04) Pierella Karlusich, Juan J.; Matias D. Zurbriggen, Matias D.; Shahinnia, Fahimeh; Sonnewald, Sophia; Sonnewald, Uwe; Hosseini, Seyed A.; Hajirezaei, Mohammad-Reza; Carrillo, Néstor
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    Targeting of flavodoxin to chloroplasts of mesophyll but not bundle sheath maize cells confers increased drought tolerance
    (Wiley, 2023-10-09) Demarchi, Mariana; Arce, Rocío C.; Campi, Mabel; Pierella Karlusich, Juan J.; Hajirezaei, Mohammad-Reza; Melzer, Michael; Lodeyro, Anabella F.; Chan, Raquel L.; Carrillo, Néstor; https://orcid.org/0009-0009-2702-6183; https://orcid.org/0000-0001-6549-6316; https://orcid.org/0009-0002-2997-9753; https://orcid.org/0000-0003-1739- 4424; https://orcid.org/0000-0002- 9537-0121; https://orcid.org/0000-0002-5213-4030; https://orcid.org/0000-0001-9208-2194; https://orcid.org/0000-0002-3264-0008; https://orcid.org/0000-0002-8612-5503; Dra. Maurino, Verónica
    Abiotic stresses, especially drought, represent the main factor limiting agricultural productivity world-wide. Maize, one of the top crops in terms of food, feed and biofuel production, is a C4 summer monocotyledoneous species grown as a single-cross hybrid displaying high heterosis (conducive to improved grain yield), and high sensitivity to drought stress at different growth stages (Sheoran et al., 2022). Photosynthesis is an early target of most environmental hardships including drought, which inhibits CO2 assimilation associated with stomatal closing. Under such conditions, reducing power generated by the photosynthetic electron transport chain (PETC) cannot be used in the regenerative step of the Calvin cycle, leading to the accumulation of NADPH, over-reduction of the PETC and downregulation of ferredoxin levels (Tognetti et al., 2006). The excess of excitation energy and reducing equivalents are delivered to adventitious acceptors, mostly O2, with the generation of partially reduced and/or activated energy-rich reactive oxygen species (ROS) such as the superoxide anion radical, hydrogen peroxide and singlet oxygen, which act both as toxic compounds and signaling intermediates (Foyer et al., 2017; Dvořák et al., 2021). Oxidative stress is thus an almost universal outcome of environmental adversities. [...]
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    ÍtemAcceso Abierto
    Transcriptional and metabolic profiling of potato plants expressing a plastid-targeted electron shuttle reveal modulation of genes associated to drought tolerance by chloroplast redox poise
    (MDPI, 2020-09-29) Pierella Karlusich, Juan J.; Arce, Rocío C.; Shahinnia, Fahimeh; Sonnewald, Sophia; Sonnewald, Uwe; Zurbriggen, Matias D.; Hajirezaei, Mohammad-Reza; Carrillo, Néstor; https://orcid.org/0000-0003-1739-4424; https://orcid.org/0000-0001-6549-6316; https://orcid.org/0000-0001-8236-7647; https://orcid.org/0000-0002-9185-6255
    Water limitation represents the main environmental constraint affecting crop yield worldwide. Photosynthesis is a primary drought target, resulting in over-reduction of the photosynthetic electron transport chain and increased production of reactive oxygen species in plastids. Manipulation of chloroplast electron distribution by introducing alternative electron transport sinks has been shown to increase plant tolerance to multiple environmental challenges including hydric stress, suggesting that a similar strategy could be used to improve drought tolerance in crops. We show herein that the expression of the cyanobacterial electron shuttle flavodoxin in potato chloroplasts protected photosynthetic activities even at a pre-symptomatic stage of drought. Transcriptional and metabolic profiling revealed an attenuated response to the adverse condition in flavodoxin-expressing plants, correlating with their increased stress tolerance. Interestingly, 5–6% of leaf-expressed genes were affected by flavodoxin in the absence of drought, representing pathways modulated by chloroplast redox status during normal growth. About 300 of these genes potentially contribute to stress acclimation as their modulation by flavodoxin proceeds in the same direction as their drought response in wild-type plants. Tuber yield losses under chronic water limitation were mitigated in flavodoxin-expressing plants, indicating that the flavoprotein has the potential to improve major agronomic traits in potato.