Examinando por Autor "Krink, Nicolas"

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    Disentangling the regulatory response of Agrobacterium tumefaciens CHLDO to glyphosate for engineering whole-cell phosphonate biosensors
    (American Chemical Society, 2024-09-30) Masotti, Fiorella; Krink, Nicolas; Lencina, Nicolas; Gottig, Natalia; Ottado, Jorgelina; Nikel, Pablo I.; https://orcid.org/0000-0002-9313-7481
    Phosphonates (PHTs), organic compounds with a stable C−P bond, are widely distributed in nature. Glyphosate (GP), a synthetic PHT, is extensively used in agriculture and has been linked to various human health issues and environmental damage. Given the prevalence of GP, developing cost-effective, onsite methods for GP detection is key for assessing pollution and reducing exposure risks. We adopted Agrobacterium tumefaciens CHLDO, a natural GP degrader, as a host and the source of genetic parts for constructing PHT biosensors. In this bacterial species, the phn gene cluster, encoding the C−P lyase pathway, is regulated by the PhnF transcriptional repressor. We selected the phnG promoter, which displays a dose-dependent response to GP, to build a set of whole-cell biosensors. Through stepwise genetic optimization of the transcriptional cascade, we created a whole-cell biosensor capable of detecting GP in the 0.25−50 μM range in various samples, including soil and water.