Examinando por Autor "Di Domenica, Violeta"

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    Identification and characterization of novel alphacoronaviruses in Tadarida brasiliensis (Chiroptera, Molossidae) from Argentina: insights into recombination as a mechanism favoring bat coronavirus cross-species transmission
    (ASM, 2023-09-11) Cerri, Agustina; Bolatti, Elisa María; Zorec, Tomaz M.; Montani, Maria E.; Rimondi, Agustina; Hosnjak, Lea; Casal, Pablo E.; Di Domenica, Violeta; Barquez, Rubén M.; Poljak, Mario; Giri, Adriana Angélica; https://orcid.org/0000-0003-4925-9075; https://orcid.org/0000-0001-6467-0650
    Bats are reservoirs of various coronaviruses that can jump between bat species or other mammalian hosts, including humans. This article explores coronavirus infection in three bat species (Tadarida brasiliensis, Eumops bonariensis, and Molossus molossus) of the family Molossidae from Argentina using whole viral metagenome analysis. Fecal samples of 47 bats from three semiurban or highly urbanized areas of the province of Santa Fe were investigated. After viral particle enrichment, total RNA was sequenced using the Illumina NextSeq 550 instrument; the reads were assembled into contigs and taxonomically and phylogenetically analyzed. Three novel complete Alphacoronavirus (AlphaCoV) genomes (Tb1–3) and two partial sequences were identified in T. brasiliensis (Tb4–5), and an additional four partial sequences were identified in M. molossus (Mm1–4). Phylogenomic analysis showed that the novel AlphaCoV clustered in two different lineages distinct from the 15 officially recognized AlphaCoV subgenera. Tb2 and Tb3 isolates appeared to be variants of the same virus, probably involved in a persistent infectious cycle within the T. brasiliensis colony. Using recombination analysis, we detected a statistically significant event in Spike gene, which was reinforced by phylogenetic tree incongruence analysis, involving novel Tb1 and AlphaCoVs identified in Eptesicus fuscus (family Vespertilionidae) from the U.S. The putative recombinant region is in the S1 subdomain of the Spike gene, encompassing the potential receptor-binding domain of AlphaCoVs. This study reports the firstfirstAlphaCoV genomes in molossids from the Americas and provides new insights into recombination as an important mode of evolution of coronaviruses involved in cross-species transmission.
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    Viral Metagenomic Data Analyses of Five New World Bat Species from Argentina: Identification of 35 Novel DNA Viruses
    (MDPI, 2022-01-24) Bolatti, Elisa María; Viarengo, Gastón; Zorec, Tomaž M; Cerri, Agustina; Montani, María E.; Hošnjak, Lea; Casal, Pablo E.; Bortolotto, Eugenia; Di Domenica, Violeta; Chouhy, Diego; Allasia, María Belén; Barquez, Rubén M.; Poljak, Mario; Giri, Adriana Angélica