Examinando por Autor "Cremaschi, Graciela Alicia"

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    ÍtemAcceso Abierto
    A penicillin derivative exerts an anti-metastatic activity in melanoma cells through the downregulation of integrin αvβ3 and Wnt/β-Catenin pathway
    (Frontiers Media, 2020-02-25) Barrionuevo, Elizabeth; Cayrol, María Florencia; Cremaschi, Graciela Alicia; Cornier, Patricia Griselda; Boggián, Dora Bernarda; Delpiccolo, Carina M. L.; Mata, Ernesto Gabino; Roguin, Leonor P.; Blank, Viviana C.
    The synthetic triazolylpeptidyl penicillin derivative, named TAP7f, has been previously characterized as an effective antitumor agent in vitro and in vivo against B16-F0 melanoma cells. In this study, we investigated the anti-metastatic potential of this compound on highly metastatic murine B16-F10 and human A375 melanoma cells. We found that TAP7f inhibited cell adhesion, migration and invasion in a dose-dependent manner. Additionally, we demonstrated that TAP7f downregulated integrin αvβ3 expression and Wnt/β-catenin pathway, a signaling cascade commonly related to tumor invasion and metastasis. Thus, TAP7f reduced both the enzymatic activity and the expression levels of matrix-metalloproteinases-2 and -9 in a time dependent manner. Moreover, TAP7f inhibited the expression of the transcription factor Snail and the mesenchymal markers vimentin, and N-cadherin, and up-regulated the expression of the epithelial marker E-cadherin, suggesting that the penicillin derivative affects epithelial–mesenchymal transition. Results obtained in vitro were supported by those obtained in a B16-F10-bearing mice metastatic model, that showed a significant TAP7f inhibition of lung metastasis. These findings suggest the potential of TAP7f as a chemotherapeutic agent for the treatment of metastatic melanoma.
  • Miniatura
    ÍtemAcceso Abierto
    Editorial: Health-stress-disease triangle. Pathophysiological focus and perspectives
    (Frontiers Media, 2024-05-17) Capurro, Claudia; Sobrevia, Luis; Larocca, María Cecilia; Cremaschi, Graciela Alicia; Vila Petroff, Martin
    This Frontiers Research Topic emerged as an outcome of the 2022 annual joint meeting of the Argentinean Society of Physiology (SAFIS) and the Latin American Association of Physiological Sciences (ALACF). Targeted to highlight physiological and pathophysiological studies focused on the interrelation between health, stress and disease and the molecular pathways that either favour, break or reverse this unidirectional path. The articles published herein reveal groundbreaking research on the response of the cardiovascular system to diverse stress signals.