Examinando por Autor "Acosta, Andrea"

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    Estudio preliminar de los efectos de las radiaciones gamma sobre los glóbulos rojos humanos
    (Asociación Física Argentina, 2020-07) Estrada, Ezequiel; Castellini, Horacio V.; Acosta, Andrea; Di Tullio, Liliana; Borraz, Javier; Chinellato, Alejandro; Tack, Ivan; D’Arrigo, Mabel; Riquelme, Bibiana Doris; Galassi, Mariel Elisa
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    ÍtemDesconocido
    Estudio preliminar del efecto de las radiaciones gamma sobre la concentración de iones plasmáticos en unidades transfusionales
    (Asociación Física Argentina, 2024-03-28) Alet, Analía Inés; Porini, Sabrina; Detarsio, Germán; Aresi, Ariel; Di Tullio Budassi, Liliana; Manzelli, Nestor; Acosta, Andrea; Galassi, Mariel E.; Riquelme, Bibiana Doris
    Los concentrados de glóbulos rojos son el componente que se utiliza con mayor frecuencia en las transfusiones sanguíneas. La irradiación gamma de la unidad transfusional está indicada cuando un paciente corre el riesgo de desarrollar la enfermedad de injerto contra huésped asociada a una transfusión. Sin embargo, esta irradiación exacerba la lesión de almacenamiento aumentando los niveles plasmáticos de potasio y disminuyendo los de sodio. Estos efectos sobre los glóbulos rojos reducen el tiempo de viabilidad de las unidades transfusionales irradiadas almacenadas. En este trabajo, estudiamos el efecto de diferentes dosis de radiación gamma sobre la concentración de los iones plasmáticos en las bolsas irradiadas, los cuales son indicativos de posibles alteraciones de las membranas de los glóbulos rojos y de los leucocitos. El análisis de los resultados obtenidos muestra que la radiación, a las dosis estudiadas, no afecta el consumo de glucosa. Sin embargo, la permeabilidad de la membrana de los eritrocitos aumenta con la dosis de radiación, indicando que este tipo de alteraciones podrían ser dosis dependiente. Estos resultados podrían ayudar a diseñar nuevos medios de conservación para reducir el daño a la membrana inducido por la radiación.
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    ÍtemDesconocido
    Factors associated with mortality among hospitalized patients with COVID-19 disease treated with convalescent plasma
    (American Society for Microbiology, 2023-11-08) Perichon, Armando M.; Acosta, Andrea; Di Tullio, Liliana; Munuce, María José; Pezzotto, Stella; Bottasso, Oscar; http://orcid.org/0000-0002-8472-7965
    The use of convalescent plasma (CP) for hospitalized patients with SARSCoV-2 infection might be a useful option in certain settings. Soon after the outbreak of COVID-19, the National Ministry of Health of Argentina recommended the use of CP transfusion for hospitalized patients with COVID-19 disease. Between 1 June and 3 October 2020, 480 patients, excluding those on invasive mechanical ventilation (IMV), received at least one CP infusion in the province of Santa Fe. We aimed to find factors associated with mortality among this cohort of patients. The median age was 60 years (interquartile range: 49–69 years) and 320 (66.7%) were males. Most of these patients (93.75%) received a single CP infusion, 82.1% and 95.6% before day 4 and day 7 of hospitalization, respectively. Anti-SARS-CoV-2 titers were determined in the CP units administered using Elecsys Anti-SARS-CoV-2 S assay. At 28 days of follow-up, 250 patients were discharged (52.1%), 131 (27.3%) remained hospitalized without and 16 (3.3%) with oxygen requirement, 27 (5.6%) were on IMV, and 56 (11.7%) had died. In the multivariate logistic regression analysis, the factors significantly associated with 28-day mortality were (i) requirement of IMV, (ii) the administration of CP after the third day of hospitalization, (iii) age, and (iv) number of comorbidities. The qualitative and quantitative analyses of antibodies against SARS-CoV-2 in the infused CP were not associated with mortality. Our findings may imply a seemingly favorable effect of CP administration among patients with severe COVID-19 disease when infused sooner after hospitalization.
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    ÍtemAcceso Abierto
    Preliminary study of alterations in human red blood cells by irradiation with high energy photons
    (Blucher, 2019) Riquelme, Bibiana Doris; Estrada, Ezequiel; Castellini, Horacio V.; Acosta, Andrea; Chinelatto, Alejandro; Tack, Ivan; Borraz, Javier; Di Tullio, Liliana; Galassi, Mariel Elisa; Sociedade Brasileira de Biofísica; D'Arrigo, Mabel. Colaboración en preparación de muestras biológicas; Castellani, Daniel. Colaboración en la fabricación del dispositivo adaptador de muestra.
    Introduction: Transfusion-associated graft-versus-host disease can be prevented by treating cellular blood products with gamma irradiation. A wide range of gamma irradiation dose levels are used in routine practice, but gamma irradiation dose of 25 Gy may be required to completely inactivate T cells in Red Blood Cells (RBC) units (Pelszynski, M. et al., 1994). This process decreases the survival of the RBC transfused, so it is crucial to understand the alterations caused by gamma irradiation to the erythrocyte membrane. In previous works, the biochemical and hematological effects of gamma irradiation at different storage periods were studied. It was observed that irradiation of the erythrocytes increases red cells hemolysis and leakage of intracellular potassium (Adams, F. et al., 2015; Yousuf, R. et al., 2018). The mechanisms through which irradiation causes the loss of RBC viability could be related to the primary effects of radiation. Gamma and X-ray Ionizing radiation cause indirect damage through the reactive oxygen species generated by water radiolysis (Anand, A.J. et al., 1997). The reduced deformability of RBC after irradiation could be related to the interaction of the oxygen-derived radicals with the membranes, affecting their mechanical properties and leading to deformability impairment (Kim, Y.-K. et al., 2008). In a recent work (AlZahrani K. et al., 2017), nanoestructural changes in the RBC membrane at different doses of gamma irradiation were observed using atomic force microscopy. The images shown that the roughness of the cell membrane increased dramatically with increasing doses, affecting their biophysical properties. However, more research is needed to understand the effects of gamma irradiation on the mechanical and adhesion properties of RBC. For this reason, in the present work we set out to measure the mechanical and aggregation parameters of human red blood cells exposed to gamma photons in different doses in order to determine the possible alterations due to radiation.