FCM - Secretaría de Ciencia, Tecnología e Innovación

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https://rephip.unr.edu.ar/handle/2133/13483

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Examinando FCM - Secretaría de Ciencia, Tecnología e Innovación por Autor "Bottasso, Oscar"

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    Aspectos básicos para la realización de una investigación clínica
    (Federación Argentina de Cardiología, 2013-05) Bottasso, Oscar
    Los orígenes de la investigación clínica se remontan a muchos siglos atrás. La Medicina siempre trató de aliviar el sufrimiento humano y la exploración de sus causas esta esencialmente ligada a la búsqueda de una cura o estrategias de prevención. A tal efecto, la investigación clínica hace uso de muchos métodos cuantitativos que se solapan en gran medida con aquellos utilizados en epidemiología, bioestadística y otros campos referidos a un problema clínico en particular. A partir de la descripción de la enfermedad, los pasos siguientes serán identificar causas, como así también desarrollar herramientas diagnósticas y compuestos terapéuticos. En esencia la investigación clínica puede ser considerar como una ciencia básica de la Medicina.
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    ÍtemAcceso Abierto
    Características clínico-epidemiológicas de la estrongiloidiasis en pacientes portadores de co-morbilidades
    (Sociedad Chilena de Infectología, 2017-02) Regueira Fernandes, Amanda; Romero, Sebastián; Alcântara de Souza Melo, Paula Fernanda; Ramos Araújo, Paulo Sérgio; Bottasso, Oscar; Rocha, Abraham; Brandão, Eduardo
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    ÍtemAcceso Abierto
    El Giulio Cesare que Verdi nunca compuso
    (Revista de la Facultad de Ciencias Médicas. Universidad Nacional de Rosario.) Bottasso, Oscar
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    ÍtemAcceso Abierto
    Short treatment with the tumour necrosis factor-α blocker infliximab diminishes chronic chagasic myocarditis in rats without evidence of Trypanosoma cruzi reactivation
    (British Society for Immunology, 2009-08) Pérez, Ana Rosa; Fontenella, Germán Héctor; Nocito, Ana Lía; Revelli, Silvia; Bottasso, Oscar
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    TNF-α Is Involved in the Abnormal Thymocyte Migration during Experimental Trypanosoma cruzi Infection and Favors the Export of Immature Cells
    (PLOS (Public Library of Science), 2012-03-26) Pérez, Ana Rosa; Berbert, Luiz Ricardo; Lepletier, Ailin; Revelli, Silvia; Bottasso, Oscar; Silva-Barbosa, Suse Dayse; Savino, Wilson
    Previous studies revealed a significant production of inflammatory cytokines together with severe thymic atrophy and thymocyte migratory disturbances during experimental Chagas disease. Migratory activity of thymocytes and mature T cells seem to be finely tuned by cytokines, chemokines and extracellular matrix (ECM) components. Systemic TNF-α is enhanced during infection and appears to be crucial in the response against the parasite. However, it also seems to be involved in disease pathology, since it is implicated in the arrival of T cells to effector sites, including the myocardium. Herein, we analyzed the role of TNF-α in the migratory activity of thymocytes in Trypanosoma cruzi (T. cruzi) acutely-infected mice. We found increased expression and deposition of TNF-α in the thymus of infected animals compared to controls, accompanied by increased co-localization of fibronectin, a cell migration-related ECM molecule, whose contents in the thymus of infected mice is also augmented. In-vivo studies showed an enhanced export of thymocytes in T. cruzi-infected mice, as ascertained by intrathymic injection of FITC alone or in combination with TNF-α. The increase of immature CD4+CD8+ T cells in secondary lymphoid organs was even more clear-cut when TNF-α was co-injected with FITC. Ex-vivo transmigration assays also revealed higher number of migrating cells when TNF-α was added onto fibronectin lattices, with higher input of all thymocyte subsets, including immature CD4+CD8+. Infected animals also exhibit enhanced levels of expression of both mRNA TNF-α receptors in the CD4+CD8+ subpopulation. Our findings suggest that in T. cruzi acute infection, when TNF-α is complexed with fibronectin, it favours the altered migration of thymocytes, promoting the release of mature and immature T cells to different compartments of the immune system. Conceptually, this work reinforces the notion that thymocyte migration is a multivectorial biological event in health and disease, and that TNF-α is a further player in the process.