(FBIOyF) Departamento de Ciencias Fisiológicas - Artículo de Revista
URI permanente para esta colección
Examinar
Examinando (FBIOyF) Departamento de Ciencias Fisiológicas - Artículo de Revista por Autor "Capiglioni, Alejo M."
Mostrando 1 - 5 de 5
Resultados por página
Opciones de ordenación
Ítem Acceso Abierto Adenoviral transfer of human Aquaporin-8 gene to mouse liver improves ammonia-derived ureagenesis(MDPI, 2023-06-02) Capiglioni, Alejo M.; Capitani, María Celeste; Marrone, Julieta; Marinelli, Raúl A.We previously reported that, in cultured hepatocytes, mitochondrial aquaporin-8 (AQP8) channels facilitate the conversion of ammonia to urea and that the expression of human AQP8 (hAQP8) enhances ammonia-derived ureagenesis. In this study, we evaluated whether hepatic gene transfer of hAQP8 improves detoxification of ammonia to urea in normal mice as well as in mice with impaired hepatocyte ammonia metabolism. A recombinant adenoviral (Ad) vector encoding hAQP8, AdhAQP8, or a control Ad vector was administered via retrograde infusion into the bile duct of the mice. Hepatocyte mitochondrial expression of hAQP8 was confirmed using confocal immunofluorescence and immunoblotting. The normal hAQP8-transduced mice showed decreased plasma ammonia and increased liver urea. Enhanced ureagenesis was confirmed via the NMR studies assessing the synthesis of 15N-labeled urea from 15N-labeled ammonia. In separate experiments, we made use of the model hepatotoxic agent, thioacetamide, to induce defective hepatic metabolism of ammonia in mice. The adenovirus-mediated mitochondrial expression of hAQP8 was able to restore normal ammonemia and ureagenesis in the liver of the mice. Our data suggest that hAQP8 gene transfer to mouse liver improves detoxification of ammonia to urea. This finding could help better understand and treat disorders with defective hepatic ammonia metabolism.Ítem Acceso Abierto Attenuation of liver cancer development by oral glycerol supplementation in the rat(Springer, 2017-03-02) Capiglioni, Alejo M.; Lorenzetti, Florencia; Quiroga, Ariel Darío; Parody, Juan Pablo; Ronco, María Teresa; Pisani, Gerardo Bruno; Carrillo, María Cristina; Ceballos Mancini, María Paula; Alvarez, María de Luján; Rassetto, Mauricio: asistencia técnicaÍtem Acceso Abierto Cholesterol can modulate mitochondrial aquaporin-8 expression in human hepatic cells(Wiley, 2017-03-20) Danielli, Mauro; Capiglioni, Alejo M.; Marrone, Julieta; Calamita, Giuseppe; Marinelli, Raúl A.Hepatocyte mitochondrial aquaporin-8 (mtAQP8) works as a multifunctional membrane channel protein that facilitates the uptake of ammonia for its detoxification to urea as well as the mitochondrial release of hydrogen peroxide. Since early oligonucleotide microarray studies in liver of cholesterol-fed mice showed an AQP8 downregulation, we tested whether alterations of cholesterol content per se modulate mtAQP8 expression in human hepatocyte-derived Huh-7 cells. Cholesterol loading with methyl-β-cyclodextrin (mβCD):cholesterol complexes downregulated the proteolytic activation of cholesterol-responsive sterol regulatory element-binding protein (SREBP) transcriptions factors 1 and 2, and the expression of the target gene 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR). Under such conditions, mtAQP8 mRNA and protein expressions were significantly reduced. In contrast, cholesterol depletion using mβCD alone increased SREBP-1 and 2 activation and upregulated HMGCR and mtAQP8 mRNA and protein expressions. The results suggest that cholesterol can regulate transcriptionally human hepatocyte mtAQP8 expression likely via SREBPs. The functional implications of our findings are discussed.Ítem Acceso Abierto Data of H2O2 release from AQP8-knockdown rat hepatocyte mitochondria(Elsevier, 2019-03-06) Danielli, Mauro; Marrone, Julieta; Capiglioni, Alejo M.; Marinelli, Raúl A.Ítem Acceso Abierto Data of ureagenesis from ammonia, glutamine and alanine, and mitochondrial aquaporin-8 expression in thioacetamide-treated hepatocytes(Elsevier, 2020-05) Capiglioni, Alejo M.; Alvarez, María de Luján; Marinelli, Raúl A.We present data about the synthesis of urea from different substrates, i.e., free ammonia, glutamine and alanine in primary cultured rat hepatocytes treated or untreated with the model hepatotoxic agent thioacetamide (TAA). We also provide data about the expression of mitochondrial aquaporin-8 (mtAQP8), a hepatocyte channel protein which facilitates ammonia diffusion into mitochondria to supply the urea cycle. Ammonia-derived ureagenesis was significantly inhibited by about 30% while that from the both amino acids resulted unaffected in TAA-treated hepatocytes. Protein expression of mtAQP8 was decreased by about 80% after TAA treatment. These data can be useful for the understanding of the mechanisms of drug-induced hepatic dysfunction.