2024-10-312024-10-312019-06-01Rosano, German Leandro; Morales, Enrique Salvador; Ceccarelli, Eduardo Augusto; New tools for recombinant protein production in Escherichia coli : A 5‐year update; John Wiley & Sons Inc; Protein Science; 28; 8; 7-2019; 1412-14221469-896Xhttps://hdl.handle.net/2133/28014The production of proteins in sufficient amounts is key for their study or use asbiotherapeutic agents. Escherichia coli is the host of choice for recombinant protein production givenits fast growth, easy manipulation, and cost-effectiveness. As such, its protein production capabilitiesare continuously being improved. Also, the associated tools (such as plasmids and cultivationconditions) are subject of ongoing research to optimize product yield. In this work, we review thelatest advances in recombinant protein production in E. coli.1412-1422enopenAccessEscherichia coliE. coli strainsRecombinant protein productionExpression plasmidAffinity tagsFusion partnersInclusion bodiesarticuloWileyUniversidad Nacional de RosarioAttribution-NonCommercial-NoDerivatives 4.0 International