2024-06-052024-06-052014-120074-0276https://hdl.handle.net/2133/27228We present here three expression plasmids for Trypanosoma cruzi adapted to the Gateway® recombination cloning system. Two of these plasmids were designed to express trypanosomal proteins fused to a double tag for tandem affinity purification (TAPtag). The TAPtag and Gateway® cassette were introduced into an episomal (pTEX) and an integrative (pTREX) plasmid. Both plasmids were assayed by introducing green fluorescent protein (GFP) by recombination and the integrity of the double-tagged protein was determined by western blotting and immunofluorescence microscopy. The third Gateway adapted vector assayed was the inducible pTcINDEX. When tested with GFP, pTcINDEX-GW showed a good response to tetracycline, being less leaky than its precursor (pTcINDEX).1-5enopenAccessTrypanosoma cruziExpression vectorsGateway® systemTandem affinity purificationpTcINDEXConstruction of three new Gateway® expression plasmids for Trypanosoma cruziarticuloAlonso, Victoria LucíaRitagliati, CarlaCribb, PamelaSerra, Esteban CarlosUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y FarmacéuticasAttribution-NonCommercial 4.0 International1678-8060