2021-12-212021-12-212021-10-301095-6433http://hdl.handle.net/2133/22972During diabetes, the characteristic hyperglycemia can induce red blood cell glycation. Several researchers have proposed different protocols to perform an in vitro model to study this phenomenon. In this article, some of the most important in vitro glycation protocols available in the bibliography were compared to each other. The incubation parameters as the suspension medium, glucose concentration, red blood cell concentration, time, and temperature were analyzed. Also, several assays were carried out in our laboratory, and glycated hemoglobin, erythrocyte aggregation and viscoelasticity were determined for the protocol validation. Based on the bibliographic analysis and our experimental results, an optimal protocol for in vitro glycation of red blood cells is presented.application/pdf111109engembargoedAccessHigh blood sugarNon-enzymatic glycationIn vitro protocolGlucose incubationErythrocyte glycationarticleElsevierUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas.Batista da Silva, Marcus V.Alet, Analía I.Castellini, Horacio V.Riquelme, Bibiana DorisAtribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)