2025-04-012025-04-012025-03-20Assis, R. de AB, Ferreira, RM, de Oliveira, ACP, Carvalho, FMS, Ferro, JA, de Souza, RF, Orellano, EG, Almeida, NF, García, CCM, Dandekar, AM, Chakraborty, S., Varani, AM y Moreira, LM (2025). Exploración de proteínas multitarea en secretomas de Xanthomonas: conocimientos sobre los mecanismos de las interacciones entre plantas y patógenos. Heliyon, 11(6), e42979. https://doi.org/10.1016/j.heliyon.2025.e429792405-8440https://hdl.handle.net/2133/29151Recent advances in large-scale functional genomic analysis have significantly increased interest in multitask proteins. The role of these proteins in Xanthomonas phytopathogens, a model for plantpathogen interaction studies, remains largely underexplored. In this study, we introduce an innovative systematic comparative analysis of secretomes from 18 different Xanthomonas species, integrating data from multiple proteomic studies to identify potential multitasking proteins. This approach led to the identification of 93 proteins primarily involved in central metabolism that are secreted under various physiological conditions, including 16 previously characterized moonlighting proteins. Promiscuity analysis of five selected enzymes revealed that three (asparaginase, chorismate mutase, and phosphoenolpyruvate synthase) exhibit high potential for catalyzing reactions with non-canonical substrates, suggesting additional functional roles beyond their primary enzymatic activities. Additionally, we re-annotated previously hypothetical secreted proteins, assigning functions related to central metabolism and indicating a high potential for promiscuous activity. This comprehensive compilation of potential moonlighting and promiscuous proteins in Xanthomonas provides new insights into the molecular mechanisms driving plantpathogen interactions and establishes a foundation for future experimental validations of these1-23enopenAccessPlant-pathogen interactionsMoonlight proteinsPromiscuous proteinsMultitask proteinsComparative secretomeAdaptationarticuloDe Almeida Barbosa Assis, RenataMarini Ferreira, RafaelPaulino de Oliveira, Amanda CarolinaSouza Carvalho, Flávia MariaAparecido Ferro, JesusDe Souza, Robson FranciscoOrellano, Elena G.Almeida, Nalvo FrancoGarcía Machado, Camila CarriãoDandekar, Abhaya M.Chakraborty, SandeepVarani, Alessandro M.Moreira, Leandro MarcioUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y FarmacéuticasAttribution 4.0 International