2018-12-112018-12-112012-07-011415-4757http://hdl.handle.net/2133/13785In previous studies we reported the identification of several AFLP, RAPD and RFLP molecular markers linked to apospory in Paspalum notatum . The objective of this work was to sequence these markers, obtain their flanking re -gions by chromosome walking and perform an in silico mapping analysis in rice and maize. The methylation status of two apospory-related sequences was also assessed using methylation-sensitive RFLP experiments. Fourteen mo -lecular markers were analyzed and several protein-coding sequences were identified. Copy number estimates and RFLP linkage analysis showed that the sequence PnMAI3 displayed 2-4 copies per genome and linkage to apospory. Extension of this marker by chromosome walking revealed an additional protein-coding sequence map- ping in silico in the apospory-syntenic regions of rice and maize. Approximately 5 kb corresponding to different mark- ers were characterized through the global sequencing procedure. A more refined analysis based on sequence information indicated synteny with segments of chromosomes 2 and 12 of rice and chromosomes 3 and 5 of maize. Two loci associated with apomixis locus were tested in methylation-sensitive RFLP experiments using genomic DNA extracted from leaves. Although both target sequences were methylated no methylation polymorphisms associated with the mode of reproduction were detected.application/pdf827-837engopenAccessapomixischromosome walkinggene mappingmolecular markersarticleAutoresCreative Commons Legal Code Attribute 2.5 (http://creativecommons.org/licenses/by/2.5/legalcode)