2024-03-182024-03-182014-04-171664-302Xhttps://hdl.handle.net/2133/26795Escherichia coli is one of the organisms of choice for the production of recombinant proteins. Its use as a cell factory is well-established and it has become the most popular expression platform. For this reason, there are many molecular tools and protocols at hand for the high-level production of heterologous proteins, such as a vast catalog of expression plasmids, a great number of engineered strains and many cultivation strategies.We review the different approaches for the synthesis of recombinant proteins in E. coli and discuss recent progress in this ever-growing field.enopenAccessRecombinant protein expressionEscherichia coliExpression plasmidInclusion bodiesAffinity tagsE. coli expression strainsarticuloRosano, Germán L.Ceccarelli, Eduardo AugustoUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y FarmacéuticasAttribution 4.0 International