Preparation of monospecific anti-PAG antibodies for cattle pregnancy detection: use of synthetic peptides to improve specificity

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dc.creator Ruiz Álvarez, Jimena Inés
dc.creator Teijeiro, Juan Manuel
dc.creator Marini, Patricia Estela
dc.date.accessioned 2017-12-04T16:42:51Z
dc.date.available 2017-12-04T16:42:51Z
dc.date.issued 2011-02
dc.identifier.issn 2156-8502 es
dc.identifier.uri http://hdl.handle.net/2133/9731
dc.description Immunological methods involving pregnancy associated glycoproteins (PAG) are used for cattle pregnancy detection. The faults of these methods could be overcomed by using antibodies specific for each member of the PAG family. In order to differentiate between very similar proteins, preparation of antibodies specific for peptides is a method of choice. In this work, we summarize a series of considerations regarding peptide design and choose free access NCBI, Antigenicity Plot, EMBOSS Antigenic and Expasy tools to apply them. We design peptides specific for different reported PAG members and obtain the corresponding polyclonal antibodies for five of them. es
dc.description.sponsorship Fundación Nuevo Banco de Santa Fe, Argentina es
dc.description.sponsorship Secretaría de Estado de Ciencia, Tecnología e Innovación, Provincia de Santa Fe, Argentina es
dc.format application/pdf
dc.format.extent 84-87 es
dc.language.iso eng es
dc.publisher SciRP es
dc.rights openAccess es
dc.rights.uri http://creativecommons.org/licenses/by-nc-sa/2.5/ar/ *
dc.subject Anticuerpo es
dc.subject Péptido es
dc.subject Bovino es
dc.subject Preñez es
dc.subject Antibodies es
dc.subject Pregnancy es
dc.subject Cattle es
dc.title Preparation of monospecific anti-PAG antibodies for cattle pregnancy detection: use of synthetic peptides to improve specificity es
dc.type article
dc.type artículo
dc.type publishedVersion
dc.rights.holder SCIRP es
dc.rights.holder Ruiz Álvarez, Jimena Inés es
dc.rights.holder Teijeiro, Juan Manuel es
dc.rights.holder Marini, Patricia Estela es
dc.relation.publisherversion http://file.scirp.org/Html/4613.html es
dc.relation.publisherversion http://doi:10.4236/abb.2011.22013 es
dc.citation.title Advances in Bioscience and Biotechnology es
dc.citation.volume 2 es
dc.description.fil Fil: Ruiz Álvarez, Jimena Inés. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina es
dc.description.fil Fil: Teijeiro, Juan Manuel. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina es
dc.description.fil Fil: Marini, Patricia Estela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina Borrar seleccionados es
dc.type.collection articulo
dc.type.version publishedVersion es


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