Preparation of monospecific anti-PAG antibodies for cattle pregnancy detection: use of synthetic peptides to improve specificity
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Fecha
2011-02
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SciRP
Resumen
Descripción
Immunological methods involving pregnancy associated
glycoproteins (PAG) are used for cattle pregnancy
detection. The faults of these methods could
be overcomed by using antibodies specific for each
member of the PAG family. In order to differentiate
between very similar proteins, preparation of antibodies
specific for peptides is a method of choice. In
this work, we summarize a series of considerations
regarding peptide design and choose free access
NCBI, Antigenicity Plot, EMBOSS Antigenic and
Expasy tools to apply them. We design peptides specific
for different reported PAG members and obtain
the corresponding polyclonal antibodies for five of
them.
Palabras clave
Anticuerpo, Péptido, Bovino, Preñez, Antibodies, Pregnancy, Cattle