Molecular characterization, tissue tropism, and genetic variability of the novel Mupapillomavirus type HPV204 and phylogenetically related types HPV1 and HPV63.

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dc.creator	Chouhy, Diego
dc.creator	Bolatti, Elisa María
dc.creator	Seme, Katja
dc.creator	Poljak, Mario
dc.creator	Giri, Adriana Angélica
dc.creator	Šterbenc, Anja
dc.creator	Hošnjak, Lea
dc.creator	Oštrbenk, Anja
dc.creator	Kocjan, Boštjan J.
dc.creator	Luzar, Boštjan
dc.date.accessioned	2017-11-25T02:06:34Z
dc.date.available	2017-11-25T02:06:34Z
dc.date.issued	2017
dc.identifier.other	1932-6203
dc.identifier.uri	http://hdl.handle.net/2133/9406
dc.description	HPV204 is the only newly identified Mupapillomavirus (Mu-PV) type in more than a decade. To comprehensively characterize HPV204, we performed a detailed molecular analysis of the viral genome and evaluated its clinical relevance in comparison to the other Mu-PVs, HPV1 and HPV63. The 7,227-bp long genome of HPV204 exhibits typical genomic organization of Mu-PVs with eight open reading frames (ORFs) (E6, E7, E1, E2, E8, E4, L2, and L1). We developed three type-specific quantitative real-time PCRs and used them to test a representative collection (n = 1,006) of various HPV-associated benign and malignant neoplasms, as well as samples of clinically normal cutaneous, mucosal, and mucocutaneous origins. HPV204, HPV1, and HPV63 were detected in 1.1%, 2.7%, and 1.9% of samples tested, respectively, and were present in skin and mucosa, suggesting dual tissue tropism of all Mu-PVs. To evaluate the etiological role of Mu-PVs in the development of HPV-associated neoplasms, Mu-PV viral loads per single cell were estimated. HPV1 and HPV63 were present in high viral copy numbers in 3/43 and 1/43 cutaneous warts, respectively, and were identified as the most likely causative agents of these warts. HPV204 viral load was extremely low in a single HPV204-positive cutaneous wart (7.4 × 10−7 viral copies/cell). Hence, etiological association between HPV204 and the development of cutaneous warts could not be established. To the best of our knowledge, this is the first study to evaluate the genetic variability of Mu-PVs by sequencing complete LCR genomic regions of HPV204, HPV1, and HPV63. We detected several nucleotide substitutions and deletions within the LCR genomic regions of Mu-PVs and identified two genetic variants of HPV204 and HPV63 and five genetic variants of HPV1.	es
dc.description.sponsorship	Slovenian Research Agency (ARRS), which funded AŠ through the Young Researcher Training Program	es
dc.description.sponsorship	Slovenian-Argentinian Bilateral Project BI-AR-15-17-005	es
dc.description.sponsorship	CoheaHr Project (European Commission FP7 Program, grant number: 603019)	es
dc.format	application/pdf
dc.language.iso	eng	es
dc.publisher	PLOS	es
dc.rights	openAccess	es
dc.rights.uri	http://creativecommons.org/publicdomain/zero/1.0/	*
dc.subject	HPV204	es
dc.subject	HPV1	es
dc.subject	HPV63	es
dc.subject	Genus Mu-PV	es
dc.subject	Molecular characterization	es
dc.title	Molecular characterization, tissue tropism, and genetic variability of the novel Mupapillomavirus type HPV204 and phylogenetically related types HPV1 and HPV63.	es
dc.rights.holder	PLOS	es
dc.rights.holder	Šterbenc, Anja	es
dc.rights.holder	Hošnjak, Lea	es
dc.rights.holder	Chouhy, Diego	es
dc.rights.holder	Bolatti, Elisa María	es
dc.rights.holder	Oštrbenk, Anja	es
dc.rights.holder	Seme, Katja	es
dc.rights.holder	Kocjan, Boštjan J.	es
dc.rights.holder	Luzar, Boštjan	es
dc.rights.holder	Giri, Adriana Angélica	es
dc.rights.holder	Poljak, Mario	es
dc.relation.publisherversion	http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0175892	es
dc.citation.title	PLOS ONE
dc.citation.volume	12(4)
dc.description.fil	Fil: Chouhy, Diego. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas. Área de Virología; Argentina
dc.description.fil	Fil: Šterbenc, Anja. University of Ljubljana. Faculty of Medicine. Institute of Microbiology and Immunology; Slovenia
dc.description.fil	Fil: Hošnjak, Lea. University of Ljubljana. Faculty of Medicine. Institute of Microbiology and Immunology; Slovenia
dc.description.fil	Fil: Bolatti, Elisa María. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas. Área de Virología; Argentina
dc.description.fil	Fil: Oštrbenk, Anja. University of Ljubljana. Faculty of Medicine. Institute of Microbiology and Immunology; Slovenia
dc.description.fil	Fil: Seme, Katja. University of Ljubljana. Faculty of Medicine. Institute of Microbiology and Immunology; Slovenia
dc.description.fil	Fil: Kocjan, Boštjan J. University of Ljubljana. Faculty of Medicine. Institute of Microbiology and Immunology; Slovenia
dc.description.fil	Fil: Luzar, Boštjan. University of Ljubljana. Faculty of Medicine. Institute of Pathology; Slovenia
dc.description.fil	Fil: Giri, Adriana Angélica. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas. Área de Virología; Argentina
dc.description.fil	Fil: Poljak, Mario. University of Ljubljana. Faculty of Medicine. Institute of Microbiology and Immunology; Slovenia