Characterization of the diverse plasmid pool harbored by the blaNDM-1-containing Acinetobacter bereziniae HPC229 clinical strain
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2019-11-19
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Public Library of Science (PLOS)
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Acinetobacter bereziniae is an environmental microorganism with increasing clinical incidence, and may thus provide a model for a bacterial species bridging the gap between the
environment and the clinical setting. A. bereziniae plasmids have been poorly studied, and
their characterization could offer clues on the causes underlying the leap between these two
different habitats. Here we characterized the whole plasmid content of A. bereziniae
HPC229, a clinical strain previously reported to harbor a 44-kbp plasmid, pNDM229, confer ring carbapenem and aminoglycoside resistance. We identified five extra plasmids in
HPC229 ranging from 114 to 1.3 kbp, including pAbe229-114 (114 kbp) encoding a
MOBP111 relaxase and carrying heavy metal resistance, a bacteriophage defense BREX
system and four different toxin-antitoxin (TA) systems. Two other replicons, pAbe229-15
(15.4 kbp) and pAbe229-9 (9.1 kbp), both encoding MOBQ1 relaxases and also carrying TA
systems, were found. The three latter plasmids contained Acinetobacter Rep_3 superfamily
replication initiator protein genes, and functional analysis of their transfer regions revealed
the mobilizable nature of them. HPC229 also harbors two smaller plasmids, pAbe229-4 (4.4
kbp) and pAbe229-1 (1.3 kbp), the former bearing a ColE1-type replicon and a TA system,
and the latter lacking known replication functions. Comparative sequence analyses against
deposited Acinetobacter genomes indicated that the above five HPC229 plasmids were
unique, although some regions were also present in other of these genomes. The transfer,
replication, and adaptive modules in pAbe229-15, and the stability module in pAbe229-9,
were bordered by sites potentially recognized by XerC/XerD site-specific tyrosine recombi nases, thus suggesting a potential mechanism for their acquisition. The presence of Rep_3
and ColE1-based replication modules, different mob genes, distinct adaptive functions
including resistance to heavy metal and other environmental stressors, as well as antimicrobial resistance genes, and a high content of XerC/XerD sites among HPC229 plasmids provide evidence of substantial links with bacterial species derived from both environmental
and clinical habitats.
Para citar este articulo: Brovedan M, Repizo GD, Marchiaro P, Viale AM, Limansky A (2019) Characterization of the diverse plasmid pool harbored by the blaNDM-1- containing Acinetobacter bereziniae HPC229 clinical strain. PLoS ONE 14(11): e0220584. https://doi.org/10.1371/journal.pone.0220584
Para citar este articulo: Brovedan M, Repizo GD, Marchiaro P, Viale AM, Limansky A (2019) Characterization of the diverse plasmid pool harbored by the blaNDM-1- containing Acinetobacter bereziniae HPC229 clinical strain. PLoS ONE 14(11): e0220584. https://doi.org/10.1371/journal.pone.0220584
Palabras clave
Acinetobacter bereziniae, Plasmids, Gene Transfer, Horizontal