Identification of FadAB complexes involved in fatty acid β-oxidation in Streptomyces coelicolor and construction of a triacylglycerol overproducing strain
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2017-08-02
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Frontiers Media
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Oleaginous microorganisms represent possible platforms for the sustainable production
of oleochemicals and biofuels due to their metabolic robustness and the possibility
to be engineered. Streptomyces coelicolor is among the narrow group of prokaryotes
capable of accumulating triacylglycerol (TAG) as carbon and energy reserve. Although the
pathways for TAG biosynthesis in this organism have been widely addressed, the set of
genes required for their breakdown have remained elusive so far. Here, we identified and
characterized three gene clusters involved in the β-oxidation of fatty acids (FA). The role of
each of the three different S. coelicolor FadAB proteins in FA catabolism was confirmed
by complementation of an Escherichia coli1fadBA mutant strain deficient in β-oxidation.
In S. coelicolor, the expression profile of the three gene clusters showed variation related
with the stage of growth and the presence of FA in media. Flux balance analyses using
a corrected version of the current S. coelicolor metabolic model containing detailed
TAG biosynthesis reactions suggested the relevance of the identified fadAB genes in
the accumulation of TAG. Thus, through the construction and analysis of fadAB knockout
mutant strains, we obtained an S. coelicolor mutant that showed a 4.3-fold increase in the
TAG content compared to the wild type strain grown under the same culture conditions.
Para citar este articulo: Menendez-Bravo S, Paganini J, Avignone-Rossa C, Gramajo H and Arabolaza A (2017) Identification of FadAB Complexes Involved in Fatty Acid β-Oxidation in Streptomyces coelicolor and Construction of a Triacylglycerol Overproducing strain. Front. Microbiol. 8:1428. doi: 10.3389/fmicb.2017.01428
Para citar este articulo: Menendez-Bravo S, Paganini J, Avignone-Rossa C, Gramajo H and Arabolaza A (2017) Identification of FadAB Complexes Involved in Fatty Acid β-Oxidation in Streptomyces coelicolor and Construction of a Triacylglycerol Overproducing strain. Front. Microbiol. 8:1428. doi: 10.3389/fmicb.2017.01428
Palabras clave
Streptomyces coelicolor, Triacylglycerol Accumulation, Chemostat System, Beta Oxidation of Fatty Acids