Alterations of the erythrocyte membrane by in vitro action of Trichinella spiralis muscle larvae

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dc.creator Ponce de León, Patricia
dc.creator Bellini, Mariana
dc.creator Castellini, Horacio V.
dc.creator Riquelme, Bibiana Doris
dc.date.accessioned 2020-03-02T22:04:17Z
dc.date.available 2020-03-02T22:04:17Z
dc.date.issued 2018-11-29
dc.identifier.issn 1475-2697 es
dc.identifier.uri http://hdl.handle.net/2133/17697
dc.description The complex life cycle of Trichinella spiralis includes the migration of newborn larvae through the bloodstream to their encystment in muscle. The parasite establishes an intimate contact with the erythrocytes of the host both during the migration of the newborn larvae and when encysting, as this parasite causes intense vascularization in the muscle cell. The goal of this work was to study the effects of various concentrations of T. spiralis muscle larvae (ML) on erythrocyte membranes. The treatment was performed by incubating human erythrocytes with equal volume of different concentrations of ML for 30 minutes, with controlled agitation (37°C). The control erythrocytes (with no contact with the larvae) were incubated in the same way with an equal volume of physiological solution. To evaluate the alterations to the erythrocytes by the action of the larvae and in the respective controls, an Erythrocyte Rheometer and a Digital Image Analysis technique were used. The results indicated that when the larval concentration was higher, the aggregation and erythrocyte membrane alterations were also higher. Also, the erythrocyte deformability index and the erythrocyte elasticity increased. The values of isolated cell coefficient varied from 0.51 in the treatment with 100 larvae/ml to 0.91 in the incubation with 1000 larvae/ml. This experiment shows that T. spiralis muscle larvae affect significantly the red blood cell aggregation and the erythrocyte viscoelastic properties. es
dc.description.sponsorship Universidad Nacional de Rosario es
dc.format application/pdf
dc.format.extent E17 es
dc.language.iso eng es
dc.publisher Cambridge University Press es
dc.rights embargoedAccess es
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es es
dc.subject Erythrocyte aggregation es
dc.subject Erythrocyte viscoelasticity es
dc.subject Trichinella spiralis muscle larvae es
dc.title Alterations of the erythrocyte membrane by in vitro action of Trichinella spiralis muscle larvae es
dc.type article
dc.type artículo
dc.type acceptedVersion
dc.rights.holder Cambridge University Press es
dc.rights.holder Ponce de León, Patricia es
dc.rights.holder Bellini, Mariana es
dc.rights.holder Castellini, Horacio V. es
dc.rights.holder Riquelme, Bibiana Doris es
dc.rights.holder Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas es
dc.relation.publisherversion https://www.cambridge.org/core/journals/journal-of-helminthology/article/alterations-of-the-erythrocyte-membrane-by-in-vitro-action-of-trichinella-spiralis-muscle-larvae/B8DC0F8CE1FB5C814266CBC1B93699AA#fndtn-information es
dc.relation.publisherversion http://dx.doi.org/10.1017/S0022149X18001062 es
dc.rights.text Atribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0) es
dc.citation.title Journal of Helminthology es
dc.citation.volume 94 es
dc.description.fil Fil: Ponce de León, Patricia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. es
dc.description.fil Fil: Bellini, Mariana. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. es
dc.description.fil Fil: Castellini, Horacio V. Universidad Nacional de Rosario. Facultad de Ciencias Exactas, Ingeniería y Agrimensura; Argentina. es
dc.description.fil Fil: Riquelme, Bibiana Doris. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Física Rosario (IFIR-CONICET); Argentina. es
dc.type.collection articulo
dc.type.version acceptedVersion es


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