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Ítem Acceso Abierto Deficiencia de glucosa-6-fosfato deshidrogenasa eritrocitaria en Rosario(ederación Bioquímica de la Provincia de Buenos Aires, 2012-07) Acosta, Irma del Luján; Milani, Ángela Cristina; Pérez, Susana Mabel; Lanza, Olga; Detarsio, Germán A.Se estudió la actividad enzimática (AE) de la enzima glucosa-6-fosfato deshidrogenasa eritrocitaria (G6PD) y la movilidad electroforética (ME) en una población de hombres y mujeres de la ciudad de Rosario (provincia de Santa Fe), Argentina y zona de influencia. Para la determinación de AE se utilizó la técnica cinética de Glock y McLean y para la electroforesis de la enzima, la técnica de M.C. Rattazzi y L.C. Bernini en acetato de celulosa. Los valores normales de actividad enzimática (AE) para hombres y mujeres adultos fueron de 8,1 ± 1,4 UI G6PD/g Hb. Se demostró que los valores de AE son independientes de la edad, sexo y concentración de hemoglobina. En todos los grupos etarios estudiados no se observaron diferencias significativas de AE con respecto a los adultos normales a excepción de los neonatos que presentaron un significativo aumento de la misma, lo cual está directamente relacionado con las características fisiológicas de los eritrocitos del recién nacido. Entre los 686 individuos estudiados se detectaron 2 pacientes deficientes de G6PD, lo que dio una prevalencia de 0,3% y el patrón electroforético correspondiente a esta población fue 98% (n: 672) para G6PD B y 2% (n: 14) para G6PD con movilidad rápida tipo A.Ítem Acceso Abierto Physiological concentrations of unconjugated bilirubin prevent oxidative stress-induced hepatocanalicular dysfunction and cholestasis(Springer, 2014-02) Basiglio, Cecilia Lorena; Toledo, Flavia D.; Boaglio, Andrea C.; Arriaga, Sandra Mónica María; Ochoa, Justina E.; Sánchez Pozzi, Enrique Juan; Mottino, Aldo D.; Roma, Marcelo GabrielÍtem Acceso Abierto The Ca2+-calmodulin-Ca2+/calmodulin-dependent protein kinase II pathway is involved in oxidative stress-induced mitochondrial permeability transition and apoptosis in rat hepatocytes(Springer, 2014-03-11) Toledo, Flavia D.; Pérez, Leonardo Martín; Basiglio, Cecilia Lorena; Ochoa, Justina E.; Sánchez Pozzi, Enrique Juan; Roma, Marcelo GabrielÍtem Acceso Abierto Relationship between genotoxic effects of breast cancer treatments and patient basal DNA integrity(BEGELL HOUSE INC, 2014-04-15) Capitaine Funes, Juan; Massa, Estefanía; Cipulli, Germán; Benitez Gil, Alfonso; Capitaine Funes, Carlos; Tozzini, Roberto; Ghersevich, Sergio Albino; Ceballos Mancini, María PaulaÍtem Acceso Abierto G-protein-coupled receptor 30/adenylyl cyclase/protein kinase A pathway is involved in estradiol 17ß-D-glucuronide-induced cholestasis(Wiley, 2014-10) Zucchetti, Andrés E.; Barosso, Ismael R.; Boaglio, Andrea C.; Basiglio, Cecilia Lorena; Miszczuk, Gisel Sabrina; Larocca, María Cecilia; Ruiz, María Laura; Davio, Carlos A.; Roma, Marcelo Gabriel; Crocenzi, Fernando A.; Sánchez Pozzi, Enrique JuanÍtem Acceso Abierto Evaluation of urinary N-acetyl-beta-D-glucosaminidase as a marker of early renal damage in patients with type 2 diabetes mellitus(Sociedade Brasileira de Endocrinologia e Metabologia (SBEM), 2014-11) Bouvet, Beatriz Reina; Paparella, Cecilia Vicenta; Arriaga, Sandra Mónica María; Monje, Adriana L.; Amarilla, Ana María; Almará, Adriana MaríaÍtem Acceso Abierto Structural determinants of Arabidopsis thaliana Hyponastic Leaves 1 function in vivo(Public Library of Science, 2014-11-19) Burdisso, Paula; Milia, Fernando; Schapire, Arnaldo L.; Bologna, Nicolás G.; Palatnik, Javier F.; Rasia, Rodolfo M.MicroRNAs have turned out to be important regulators of gene expression. These molecules originate from longer transcripts that are processed by ribonuclease III (RNAse III) enzymes. Dicer proteins are essential RNAse III enzymes that are involved in the generation of microRNAs (miRNAs) and other small RNAs. The correct function of Dicer relies on the participation of accessory dsRNA binding proteins, the exact function of which is not well-understood so far. In plants, the double stranded RNA binding protein Hyponastic Leaves 1 (HYL1) helps Dicer Like protein (DCL1) to achieve an efficient and precise excision of the miRNAs from their primary precursors. Here we dissected the regions of HYL1 that are essential for its function in Arabidopsis thaliana plant model. We generated mutant forms of the protein that retain their structure but affect its RNA-binding properties. The mutant versions of HYL1 were studied both in vitro and in vivo, and we were able to identify essential aminoacids/residues for its activity. Remarkably, mutation and even ablation of one of the purportedly main RNA binding determinants does not give rise to any major disturbances in the function of the protein. We studied the function of the mutant forms in vivo, establishing a direct correlation between affinity for the pri-miRNA precursors and protein activity.Ítem Acceso Abierto Importancia de la evaluación del estrés oxidativo en el semen humano(Prensa Medica Latinoamericana, 2015-03) Paparella, Cecilia Vicenta; Pavesi, Adriana Beatriz; Feldman, Rodolfo Néstor; Bouvet, Beatriz ReinaÍtem Acceso Abierto Fibroblast growth factor receptors (FGFRs) in human sperm: expression, functionality and involvement in motility regulation(Public Library of Science (PLOS), 2015-05-13) Saucedo, Lucía; Buffa, Gabriela Natalia; Rosso, Marina; Guillardoy, Tomás; Góngora, Adrián; Munuce, María José; Vázquez Levin, Mónica Hebe; Marín Briggiler, Clara; Dr. Baldi, Alberto provide the FGF2Fibroblast growth factors receptors (FGFRs) have been widely characterized in somatic cells, but there is scarce evidence of their expression and function in mammalian gametes. The objective of the present study was to evaluate the expression of FGFRs in human male germ cells, to determine sperm FGFR activation by the FGF2 ligand and their participation in the regulation of sperm motility. The expression of FGFR1, 2, 3 and 4 mRNAs and proteins in human testis and localization of these receptors in germ cells of the seminiferous epithelium was demonstrated. In ejaculated sperm, FGFRs were localized to the acrosomal region and flagellum. Sperm exposure to FGF2 caused an increase in flagellar FGFR phosphorylation and activation of extracellular signal-regulated kinase (ERK) and protein kinase B (PKB or Akt) signaling pathways. Incubation with FGF2 led to a significant increase in the percentage of total and progressive sperm motility, as well as in sperm kinematics. All responses were prevented by sperm preincubation with BGJ398, a specific inhibitor of FGFR tyrosine kinase activity. In addition to confirming the expression of FGFRs in germ cells of the human testis, our study describes for the first time the presence, localization and functionality of human sperm FGFRs, and provides evidence of the beneficial effect of FGF2 upon sperm motility.Ítem Acceso Abierto Induced folding in RNA recognition by Arabidopsis thaliana DCL1(Oxford University Press, 2015-06-22) Suárez, Irina Paula; Burdisso, Paula; Benoit, Matthieu P. M. H.; Boisbouvier, Jérôme; Rasia, Rodolfo M.Ítem Acceso Abierto Effects of lactoferrin, a protein present in the female reproductive tract, on parameters of human sperm capacitation and gamete interaction(Wiley, 2015-10-07) Zumoffen, Carlos María; Massa, Estefanía; Caille, Adriana María; Munuce, María José; Ghersevich, Sergio AlbinoIn a recent study, lactoferrin (LF) was detected in human oviductal secretion. The protein was able to bind to oocytes and sperm, and modulated gamete interaction. The aim of the present study was to investigate the effect of LF on parameters related to human sperm capacitation and sperm–zona pellucida interaction. Semen samples were obtained from healthy normozoospermic donors (n = 7). Human follicular fluids and oocytes were collected from patients undergoing in vitro fertilization. Motile sperm obtained by swim-up were incubated for 6 or 22 h under capacitating conditions with LF (0–100 μg/mL). After incubations, viability, motility, presence of α-d-mannose receptors (using a fluorescent probe on mannose coupled to bovine serum albumin), spontaneous and induced acrosome reaction (assessed with Pisum sativum agglutinin conjugated to fluorescein isothiocyanate), and tyrosine phosphorylation of sperm proteins were evaluated. Sperm–zona pellucida interaction in the presence of LF was investigated using the hemizone assay. The presence of LF did not affect sperm viability or motility, but caused a dose-dependent significant decrease in sperm α-d-mannose-binding sites, and the effect was already significant with the lowest concentration of the protein used after 22 h incubation. Dose-dependent significant increases in both induced acrosome reaction and tyrosine phosphorylation of sperm proteins were observed in the presence of LF. The present data indicate that LF modulates parameters of sperm function. The inhibition of gamete interaction by LF could be partially explained by the decrease in sperm d-mannose-binding sites. The presence of the LF promoted sperm capacitation in vitro.Ítem Acceso Abierto P1 epigenetic regulation in leaves of high altitude maize landraces: effect of UV-B radiation(Frontiers Media, 2016-04-21) Rius, Sebastián P.; Emiliani, Julia; Casati, PaulaÍtem Acceso Abierto Differential metabolic rearrangements after cold storage are correlated with chilling injury resistance of peach fruits(Frontiers Media, 2016-09-30) Bustamante, Claudia Anabel; Monti, Laura Lucía; Gabilondo, Julieta; Scossa, Federico; Budde, Claudio O.; Lara, María Valeria; Fernie, Alisdair R.; Drincovich, María FabianaÍtem Acceso Abierto Exposición laboral a factores medioambientales y su efecto sobre parámetros seminales en hombres con infertilidad idiopática(Universidad Nacional de Rosario, 2016-10) Paparella, Cecilia Vicenta; Pavesi, Adriana Beatriz; Provenzal, Olga; Ombrella, Adriana María; Luciano, María Ángeles; Rodriguez, Andrea; Bouvet, Beatriz ReinaÍtem Acceso Abierto Antiphospholipid and antioangiogenic activity in females with recurrent miscarriage and antiphospholipid syndrome(SAGE, 2016-11-02) Pelusa, Héctor Fabián; Pezzarini, Eleonora; Basiglio, Cecilia Lorena; Musuruana, Jorge; Bearzotti, Mariela; Svetaz, María José; Daniele, Stella Maris; Bottai, Hebe; Arriaga, Sandra Mónica MaríaÍtem Acceso Abierto Acute diarrhoea in children: determination of duration using a combined bismuth hydroxide gel and oral rehydration solution therapy vs. oral rehydration solution(MDPI, 2016-12-21) Oviedo, Adriana; Díaz, Mirna; Valenzuela, María Laura; Vidal, Victoria; Racca, Liliana; Bottai, Hebe; Priore, Graciela; Peluffo, Graciela; Di Bartolomeo, Susana; Cabral, Graciela; Toca, María del CarmenOral rehydration salt (ORS) treatment in young children with acute diarrhoea (AD) has contributed to decrease mortality associated with dehydration although effective strategies to reduce morbidity associated with this disease are required. The aim of this study was to evaluate the diarrhoea duration when using combined colloidal bismuth hydroxide gel (CBHG) and oral rehydration salt treatment compared with ORS therapy in children with AD. We designed a double-blind, randomised prospective study with treatment and control groups. Patients aged one to 12 years, with no prior pathology and with AD of less than 48 h were included. The Chi-squared and Mann-Whitney tests were used, as well as the Cox proportional hazards model and the Kaplan-Meier estimator. Patients were randomised into an ORS and CBHG treatment group and a control group for ORS plus placebo. (Average age: 3.2 years). The result of the post-treatment evaluation with respect to the average duration of AD was 25.5 h for the treated group vs. 41.5 h for the control group (p = 0.015). The average number of stools was 4.8 in the treated group and 8.2 in the control group (p = 0.032). We conclude that the use of CBHG plus ORS significantly reduced the duration of AD, the number of stools and the percentage of children with persistent AD after 24 h of treatment compared to the control group. AD remitted almost twice as fast in patients treated with CBHG and ORS compared to those who received ORS plus placebo.Ítem Acceso Abierto Mitogen-activated protein kinases are involved in hepatocanalicular dysfunction and cholestasis induced by oxidative stress(Springer, 2017-06) Toledo, Flavia D.; Basiglio, Cecilia Lorena; Barosso, Ismael R.; Boaglio, Andrea C.; Zucchetti, Andrés E.; Sanchez Pozzi, Enrique J.; Roma, Marcelo GabrielÍtem Acceso Abierto Identificación de espermatozoides humanos en muestras contaminadas con levaduras(Universidad Autónoma de Tamaulipas, 2017-07) Bouvet, Beatriz Reina; Pavesi, Adriana Beatriz; Paparella, Cecilia Vicenta; Ombrella, Adriana MaríaÍtem Acceso Abierto Infertilidad masculina : Exposición laboral a factores ambientales y su efecto sobre la calidad seminal(Sociedad de Medicina Interna del Uruguay, 2017-09) Pavesi, Adriana Beatriz; Provenzal, Olga; Ombrella, Adriana María; Bouvet, Beatriz Reina; Paparella, Cecilia VicentaÍtem Acceso Abierto A general reaction mechanism for carbapenem hydrolysis by mononuclear and binuclear metallo-β-lactamases(Nature, 2017-09-14) Lisa, María Natalia; Palacios, Antonela R.; Aitha, Mahesh; González, Mariano M.; Moreno, Diego M.; Crowder, Michael W.; Bonomo, Robert A.; Spencer, James; Tierney, David L.; Llarrull, Leticia Irene; Vila, Alejandro J.