Development of a second–order standard addition fluorescence method for the direct determination of riboflavin in human urine samples without previous clean up and separation steps

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dc.creator Lozano, Valeria Antonella
dc.creator Privitera, María Laura
dc.date.accessioned 2017-12-04T21:03:04Z
dc.date.available 2017-12-04T21:03:04Z
dc.date.issued 2017-07
dc.identifier.issn 0026-265X
dc.identifier.uri http://hdl.handle.net/2133/9737
dc.description A new strategy for the determination of riboflavin (RF, or vitamin B2) in human urine samples has been developed, combining excitation–emission fluorescence matrix (EEFM) data in standard addition mode and second–order chemometric analysis. The method is simple, fast and eco–friendly because it complies with the green analytical chemistry principles, avoiding the need of previous clean up and separation steps that consume high amounts of organic solvents. Successful results were obtained by different chemometric algorithms, namely parallel factor analysis (PARAFAC), unfolded partial least–squares/residual bilinearization (U–PLS/RBL) and multidimensional partial least–squares/residual bilinearization (N–PLS/RBL), all in the modified standard addition. These algorithms allowed us to achieve selectivity in a system, which requires standard addition and shows a significant background spectral overlapping with the studied vitamin. The quality of the proposed strategy was evidenced on the basis of the analytical recoveries from urine samples spiked with RF. The detection limits achieved in urine samples are encouraging compared to those obtained using chromatographic approaches. The relative prediction errors were lower than 5.6 %. es
dc.description.sponsorship Universidad Nacional de Rosario (Proyecto BIO 374) es
dc.description.sponsorship Consejo Nacional de Investigaciones Científicas y Técnicas (Proyecto PIP 0163) es
dc.description.sponsorship Agencia Nacional de Promoción Científica y Tecnológica (Proyecto PICT 2013–0136) es
dc.format application/pdf
dc.language.iso eng es
dc.publisher Elsevier es
dc.rights openAccess es
dc.rights.uri http://creativecommons.org/licenses/by-nc-sa/2.5/ar/ *
dc.subject Human urine samples es
dc.subject Green analytical chemistry es
dc.subject Second–order standard addition es
dc.subject Fluorescence es
dc.subject Chemometrics es
dc.subject Riboflavin es
dc.title Development of a second–order standard addition fluorescence method for the direct determination of riboflavin in human urine samples without previous clean up and separation steps es
dc.rights.holder Elsevier es
dc.rights.holder Privitera, María L. es
dc.rights.holder Lozano, Valeria A. es
dc.relation.publisherversion http://www.sciencedirect.com/science/article/pii/S0026265X16303721 es
dc.relation.publisherversion https://doi.org/10.1016/j.microc.2017.02.033 es
dc.citation.title Microchemical Journal
dc.citation.volume 133
dc.description.fil Fil: Lozano, Valeria Antonella. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Química Rosario (IQUIR–CONICET); Argentina
dc.description.fil Fil: Privitera, María Laura. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Química Rosario (IQUIR–CONICET); Argentina


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