Sequence characterization, in silico mapping and cytosine methylation analysis of markers linked to apospory in Paspalum notatum

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dc.creator Podio, Maricel
dc.creator Rodríguez, María Pía
dc.creator Felitti, Silvina
dc.creator Stein, Juliana
dc.creator Martínez, Erci Javier
dc.creator Siena, Lorena Adelina
dc.creator Quarin, Camilo Luis
dc.creator Pessino, Silvina Claudia
dc.creator Ortiz, Juan Pablo Amelio
dc.date.accessioned 2018-12-11T14:13:58Z
dc.date.available 2018-12-11T14:13:58Z
dc.date.issued 2012-07-01
dc.identifier.issn 1415-4757 es
dc.identifier.uri http://hdl.handle.net/2133/13785
dc.description In previous studies we reported the identification of several AFLP, RAPD and RFLP molecular markers linked to apospory in Paspalum notatum . The objective of this work was to sequence these markers, obtain their flanking re -gions by chromosome walking and perform an in silico mapping analysis in rice and maize. The methylation status of two apospory-related sequences was also assessed using methylation-sensitive RFLP experiments. Fourteen mo -lecular markers were analyzed and several protein-coding sequences were identified. Copy number estimates and RFLP linkage analysis showed that the sequence PnMAI3 displayed 2-4 copies per genome and linkage to apospory. Extension of this marker by chromosome walking revealed an additional protein-coding sequence map- ping in silico in the apospory-syntenic regions of rice and maize. Approximately 5 kb corresponding to different mark- ers were characterized through the global sequencing procedure. A more refined analysis based on sequence information indicated synteny with segments of chromosomes 2 and 12 of rice and chromosomes 3 and 5 of maize. Two loci associated with apomixis locus were tested in methylation-sensitive RFLP experiments using genomic DNA extracted from leaves. Although both target sequences were methylated no methylation polymorphisms associated with the mode of reproduction were detected. es
dc.format application/pdf
dc.format.extent 827-837 es
dc.language.iso eng es
dc.publisher Sociedade Brasileira de Genética es
dc.rights openAccess es
dc.rights.uri https://creativecommons.org/licenses/by-nc/4.0/deed.es *
dc.subject apomixis es
dc.subject chromosome walking es
dc.subject gene mapping es
dc.subject molecular markers es
dc.title Sequence characterization, in silico mapping and cytosine methylation analysis of markers linked to apospory in Paspalum notatum es
dc.type article
dc.type artículo
dc.type publishedVersion
dc.rights.holder Autores es
dc.relation.publisherversion www.gmb.org.br es
dc.rights.text Creative Commons Legal Code Attribute 2.5 (http://creativecommons.org/licenses/by/2.5/legalcode) es
dc.citation.title Genetics and Molecular Biology es
dc.citation.volume 35 es
dc.description.fil Laboratorio de Biología Molecular, Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, Zavalla, Santa Fe, Argentina es
dc.type.collection articulo
dc.type.version publishedVersion es


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